caa a22 4500 606155333 CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10096-014-2215-7 doi (NATIONALLICENCE)springer-10.1007/s10096-014-2215-7 Epidemic and molecular evolution of human bocavirus in hospitalized children with acute respiratory tract infection [Elektronische Daten] [Q.-B. Lu, Y. Wo, H.-Y. Wang, D.-D. Huang, J. Zhao, X.-A. Zhang, Y.-Y. Zhang, E.-M. Liu, W. Liu, W.-C. Cao] Human bocavirus (HBoV) is a novel parvovirus, often associated with respiratory tract diseases in children. This study explored the epidemiological characteristics and molecular evolution of HBoV-1 in southeastern China. Nasopharyngeal aspirates were collected from children admitted to hospital with acute respiratory tract infections. HBoV-1 was detected using real-time reverse transcription polymerase chain reaction and further characterized by complete genome sequences analysis. Among the 3,022 recruited children, 386 (12.77%) were HBoV-1-positive and 300 (77.72%) had co-detection with other respiratory viruses. Seasonal prevalence peaked in summer. HBoV-1 presence was significantly associated with asthma attack [odds ratio = 1.74; 95 % confidence interval: 1.30, 2.31; p < 0.001]. Similar results were obtained when either single detection or co-detection of HBoV-1 was considered, demonstrating the minor impact of co-detection on the clinical characteristics or epidemic pattern. Phylogenetic analysis based on the complete genome sequences showed that all the HBoV-1 sequences clustered together and no branch was formed that was supported by bootstrap value ≥750. The overall evolutionary rate of the complete genome of HBoV-1 was estimated at 1.08 × 10−4 nucleotide substitutions per site per year (s/s/y) [95% highest probability density: (0.40-1.86) × 10−4s/s/y]. Selective pressure analysis showed that all the ω-values were less than 1, suggesting that HBoV-1 was under negative selective pressure. Site-by-site analysis identified the codon site 40 of the VP1 gene under positive selection. In conclusion, our study disclosed the epidemiological and genetic dynamics of HBoV-1 epidemics in southeastern China in the most recent 3years, the information of which might help to further improve our understanding of HBoV-1 infection and guide better surveillance and control strategies in the future. Springer-Verlag Berlin Heidelberg, 2014 Lu Q.-B School of Public Health, Peking University, 100191, Beijing, China aut Wo Y. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut Wang H.-Y State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut Huang D.-D State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut Zhao J. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut Zhang X.-A State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut Zhang Y.-Y State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut Liu E.-M Children's Hospital, Chongqing Medical University, 400014, Chongqing, China aut Liu W. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut Cao W.-C State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/1(2015-01-01), 75-81 0934-9723 34:1<75 2015 34 10096 https://doi.org/10.1007/s10096-014-2215-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2215-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Q.-B School of Public Health, Peking University, 100191, Beijing, China aut NATIONALLICENCE 700 1- Wo Y. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut NATIONALLICENCE 700 1- Wang H.-Y State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut NATIONALLICENCE 700 1- Huang D.-D State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut NATIONALLICENCE 700 1- Zhao J. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut NATIONALLICENCE 700 1- Zhang X.-A State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut NATIONALLICENCE 700 1- Zhang Y.-Y State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut NATIONALLICENCE 700 1- Liu E.-M Children's Hospital, Chongqing Medical University, 400014, Chongqing, China aut NATIONALLICENCE 700 1- Liu W. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut NATIONALLICENCE 700 1- Cao W.-C State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da Street, Fengtai District, 100071, Beijing, China aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/1(2015-01-01), 75-81 0934-9723 34:1<75 2015 34 10096