caa a22 4500 606155368 CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s10096-014-2216-6 doi (NATIONALLICENCE)springer-10.1007/s10096-014-2216-6 Sequence variation in virulence-related genes of Bordetella pertussis isolates from Poland in the period 1959-2013 [Elektronische Daten] [E. Mosiej, M. Zawadka, K. Krysztopa-Grzybowska, M. Polak, E. Augustynowicz, K. Piekarska, A. Lutyńska] This study aimed to characterise Bordetella pertussis isolates circulating in Poland since 1959. Sequence analysis of ptxA, ptxC, prn, tcfA, fim2, fim3 and ptxP for 175 clinical isolates and currently and previously used vaccine strains was performed. Clinical isolates from the period 1995-2013 were found to be different to three currently used vaccine strains harbouring the allelic combination ptxA2-ptxC1-ptxP1-prn1-tcfA2-fim2-1-fim3-1, seen frequently in Poland in the early pertussis vaccination period but not found after 1995. Generally, among B. pertussis isolates from the period 2000-2013, two genotypes predominated, ptxA1-ptxC1-ptxP1-prn1-tcfA2-fim2-2-fim3-1 and ptxA1-ptxC1-ptxP1-prn2-tcfA2-fim2-1-fim3-1, with frequencies of 45% and 32.5%, respectively. The isolates harbouring ptxA1-ptxC2-ptxP3-prn2-tcfA2-fim2-1-fim3-2 and ptxA1-ptxC2-ptxP3-prn2-tcfA2-fim2-1-fim3-1 profiles, currently highly prevalent within other European Union (EU) countries, were rarely found in Poland, as they circulated in the period 2000-2013 with frequencies of 10% and 5%, respectively. We hypothesise that several previous changes of strain composition in whole-cell pertussis vaccine produced locally and used since 1960 in Poland resulted in a more diverse immune pressure in the population, resulting in different prevalence of alleles compared to elsewhere. Springer-Verlag Berlin Heidelberg, 2014 Mosiej E. Department of Sera and Vaccines Evaluation, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland aut Zawadka M. Department of Sera and Vaccines Evaluation, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland aut Krysztopa-Grzybowska K. Department of Sera and Vaccines Evaluation, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland aut Polak M. Department of Sera and Vaccines Evaluation, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland aut Augustynowicz E. Department of Sera and Vaccines Evaluation, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland aut Piekarska K. Department of Bacteriology, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland aut Lutyńska A. Department of Sera and Vaccines Evaluation, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/1(2015-01-01), 147-152 0934-9723 34:1<147 2015 34 10096 https://doi.org/10.1007/s10096-014-2216-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2216-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mosiej E. Department of Sera and Vaccines Evaluation, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland aut NATIONALLICENCE 700 1- Zawadka M. Department of Sera and Vaccines Evaluation, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland aut NATIONALLICENCE 700 1- Krysztopa-Grzybowska K. Department of Sera and Vaccines Evaluation, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland aut NATIONALLICENCE 700 1- Polak M. Department of Sera and Vaccines Evaluation, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland aut NATIONALLICENCE 700 1- Augustynowicz E. Department of Sera and Vaccines Evaluation, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland aut NATIONALLICENCE 700 1- Piekarska K. Department of Bacteriology, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland aut NATIONALLICENCE 700 1- Lutyńska A. Department of Sera and Vaccines Evaluation, National Institute of Public Health—National Institute of Hygiene, Warsaw, Poland aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/1(2015-01-01), 147-152 0934-9723 34:1<147 2015 34 10096