caa a22 4500 606155449 CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10096-015-2356-3 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2356-3 Molecular epidemiology of carbapenemase-producing Escherichia coli and the prevalence of ST131 subclone H30 in Shanghai, China [Elektronische Daten] [F. Zhang, D. Zhu, L. Xie, X. Guo, Y. Ni, J. Sun] The molecular characteristics and epidemiology of carbapenemase-producing Escherichia coli (CPEC) isolates from Shanghai, China, were investigated using 21 imipenem-resistant E. coli isolates obtained from a Shanghai teaching hospital from 2011 to 2014. The presence of bla KPC, bla IMP, bla VIM, bla OXA-48, and bla NDM was assessed by polymerase chain reaction (PCR) amplification and sequencing. CPEC isolates were characterized by the Etest®, multilocus sequence typing (MLST), and pulse-field gel electrophoresis (PFGE). Plasmids carrying resistance genes were analyzed by conjugation experiments, replicon typing, plasmid MLST (pMLST), S1 nuclease PFGE (S1-PFGE), and Southern hybridization. The genetic environment of the resistance genes was determined by PCR and sequencing. Among the 21 E. coli isolates, 16 produced carbapenemases; of these, ten isolates transferred carbapenemase-encoding plasmids to recipient bacteria. Nine of the 16 isolates were clonally related, and their PFGE patterns were designated type A. ST131 was the predominant sequence type (11 isolates, 68.8%); the H30 subclone comprised 81.8% of the ST131 strains. In all three isolates, bla IMP-4 was located on 50-kb IncN plasmids. All but two bla KPC-2 genes were carried on IncF plasmids of various sizes. Hence, both clone-spread and horizontal transfer mediated the dissemination of carbapenemase-producing genes in the Shanghai isolates. Springer-Verlag Berlin Heidelberg, 2015 Zhang F. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin No. 2 Road, 200025, Shanghai, China aut Zhu D. Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, 2800 Gongwei Road, Huinan Town, Pudong, 201399, Shanghai, China aut Xie L. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin No. 2 Road, 200025, Shanghai, China aut Guo X. Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiaotong University School of Medicine, 200025, Shanghai, China aut Ni Y. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin No. 2 Road, 200025, Shanghai, China aut Sun J. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin No. 2 Road, 200025, Shanghai, China aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/6(2015-06-01), 1263-1269 0934-9723 34:6<1263 2015 34 10096 https://doi.org/10.1007/s10096-015-2356-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2356-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang F. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin No. 2 Road, 200025, Shanghai, China aut NATIONALLICENCE 700 1- Zhu D. Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, 2800 Gongwei Road, Huinan Town, Pudong, 201399, Shanghai, China aut NATIONALLICENCE 700 1- Xie L. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin No. 2 Road, 200025, Shanghai, China aut NATIONALLICENCE 700 1- Guo X. Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiaotong University School of Medicine, 200025, Shanghai, China aut NATIONALLICENCE 700 1- Ni Y. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin No. 2 Road, 200025, Shanghai, China aut NATIONALLICENCE 700 1- Sun J. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin No. 2 Road, 200025, Shanghai, China aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/6(2015-06-01), 1263-1269 0934-9723 34:6<1263 2015 34 10096