caa a22 4500 606155457 CHVBK 20210128100603.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10096-015-2350-9 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2350-9 Prevalence and distribution of beta-lactamase coding genes in third-generation cephalosporin-resistant Enterobacteriaceae from bloodstream infections in Cambodia [Elektronische Daten] [E. Vlieghe, T.-D. Huang, T. Phe, P. Bogaerts, C. Berhin, B. De Smet, W. Peetermans, J. Jacobs, Y. Glupczynski] Resistance to third-generation cephalosporins in Gram-negative bacteria is emerging in Asia. We report the prevalence and distribution of extended-spectrum beta-lactamase (ESBL), AmpC beta-lactamase and carbapenemase-coding genes in cefotaxime-resistant Enterobacteriaceae isolates from bloodstream infections (BSI) in Cambodia. All Enterobacteriaceae isolated from BSI in adult patients at Sihanouk Hospital Centre of HOPE, Phnom Penh, Cambodia (2007-2010) were assessed. Antimicrobial susceptibility testing was carried out by disc diffusion and MicroScan according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Screening for ESBL, plasmidic AmpC and carbapenemase-coding genes was performed by multiplex polymerase chain reaction (PCR) sequencing assays. Identification of the ST131 clone was performed in all CTX-M-positive Escherichia coli, using PCR targeting the papB gene. Out of 183 Enterobacteriaceae, 91 (49.7%) isolates (84 BSI episodes) were cefotaxime-resistant: E. coli (n = 68), Klebsiella pneumoniae (n = 17) and Enterobacter spp. (n = 6). Most episodes were community-acquired (66/84; 78.3%). ESBLs were present in 89/91 (97.8%) cefotaxime-resistant isolates: 86 (96.6%) were CTX-M, mainly CTX-M-15 (n = 41) and CTX-M-14 (n = 21). CTX-M of group 1 were frequently associated with TEM and/or OXA-1/30 coding genes and with phenotypic combined resistance to ciprofloxacin, sulphamethoxazole-trimethoprim and gentamicin (39/50, 78.0%). Plasmidic AmpC (CMY-2 and DHA-1 types) were found alone (n = 2) or in combination with ESBL (n = 4). Eighteen E. coli isolates were identified as B2-ST131-O25B: 11 (61.1%) carried CTX-M-14. No carbapenemase-coding genes were detected. ESBL among Enterobacteriaceae from BSI in Cambodia is common, mainly associated with CTX-M-15 and CTX-M-14. These findings warrant urgent action for the containment of antibiotic resistance in Cambodia. The Author(s), 2015 Vlieghe E. Department of Clinical Sciences, Institute of Tropical Medicine, Nationalestraat 155, 2000, Antwerp, Belgium aut Huang T.-D National Reference Laboratory for Resistance Monitoring of Gram-negative Bacteria, CHU Dinant-Godinne UCL, Avenue G. Thérasse 1, 5530, Yvoir, Belgium aut Phe T. Sihanouk Hospital Centre of HOPE, Phnom Penh, Cambodia aut Bogaerts P. National Reference Laboratory for Resistance Monitoring of Gram-negative Bacteria, CHU Dinant-Godinne UCL, Avenue G. Thérasse 1, 5530, Yvoir, Belgium aut Berhin C. National Reference Laboratory for Resistance Monitoring of Gram-negative Bacteria, CHU Dinant-Godinne UCL, Avenue G. Thérasse 1, 5530, Yvoir, Belgium aut De Smet B. Department of Clinical Sciences, Institute of Tropical Medicine, Nationalestraat 155, 2000, Antwerp, Belgium aut Peetermans W. Department of Internal Medicine, University Hospital, KU Leuven—University of Leuven, Leuven, Belgium aut Jacobs J. Department of Clinical Sciences, Institute of Tropical Medicine, Nationalestraat 155, 2000, Antwerp, Belgium aut Glupczynski Y. National Reference Laboratory for Resistance Monitoring of Gram-negative Bacteria, CHU Dinant-Godinne UCL, Avenue G. Thérasse 1, 5530, Yvoir, Belgium aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/6(2015-06-01), 1223-1229 0934-9723 34:6<1223 2015 34 10096 https://doi.org/10.1007/s10096-015-2350-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2350-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vlieghe E. Department of Clinical Sciences, Institute of Tropical Medicine, Nationalestraat 155, 2000, Antwerp, Belgium aut NATIONALLICENCE 700 1- Huang T.-D National Reference Laboratory for Resistance Monitoring of Gram-negative Bacteria, CHU Dinant-Godinne UCL, Avenue G. Thérasse 1, 5530, Yvoir, Belgium aut NATIONALLICENCE 700 1- Phe T. Sihanouk Hospital Centre of HOPE, Phnom Penh, Cambodia aut NATIONALLICENCE 700 1- Bogaerts P. National Reference Laboratory for Resistance Monitoring of Gram-negative Bacteria, CHU Dinant-Godinne UCL, Avenue G. Thérasse 1, 5530, Yvoir, Belgium aut NATIONALLICENCE 700 1- Berhin C. National Reference Laboratory for Resistance Monitoring of Gram-negative Bacteria, CHU Dinant-Godinne UCL, Avenue G. Thérasse 1, 5530, Yvoir, Belgium aut NATIONALLICENCE 700 1- De Smet B. Department of Clinical Sciences, Institute of Tropical Medicine, Nationalestraat 155, 2000, Antwerp, Belgium aut NATIONALLICENCE 700 1- Peetermans W. Department of Internal Medicine, University Hospital, KU Leuven—University of Leuven, Leuven, Belgium aut NATIONALLICENCE 700 1- Jacobs J. Department of Clinical Sciences, Institute of Tropical Medicine, Nationalestraat 155, 2000, Antwerp, Belgium aut NATIONALLICENCE 700 1- Glupczynski Y. National Reference Laboratory for Resistance Monitoring of Gram-negative Bacteria, CHU Dinant-Godinne UCL, Avenue G. Thérasse 1, 5530, Yvoir, Belgium aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/6(2015-06-01), 1223-1229 0934-9723 34:6<1223 2015 34 10096