caa a22 4500 606155600 CHVBK 20210128100604.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10096-015-2333-x doi (NATIONALLICENCE)springer-10.1007/s10096-015-2333-x Early infectious acute respiratory distress syndrome is characterized by activation and proliferation of alveolar T-cells [Elektronische Daten] [K. Risso, G. Kumar, M. Ticchioni, C. Sanfiorenzo, J. Dellamonica, F. Guillouet-de Salvador, G. Bernardin, C.-H. Marquette, P.-M. Roger] Acute respiratory distress syndrome (ARDS) in humans is characterized by the infiltration of polymorphonuclears in the alveolar spaces. However, the role of T-cells in ARDS is unknown. Our aim was to characterize the T-cell phenotype in bronchoalveolar lavage (BAL) during the early phase of acute lung infection(ALI)/ARDS-infected patients in comparison to a control group (CG). BAL lymphocyte phenotypes of two ALI, 16 ARDS, and eight CG were examined by flow cytometry. ALI/ARDS showed a significant increase in CD4 and CD8 T-cell activation as compared to CG. Moreover, a significant level of proliferation was observed using the Ki67 marker in ARDS patients as compared to controls (median): 37 versus 6% for CD4 T-cells (p = 0.022) and 34 versus 2% for CD8 T-cells (p = 0.009). In contrast, the percentage of T-regulatory cells and apoptotic T-cells were similar in both groups. Among costimulatory molecules, we observed an overexpression of CTLA-4/CD152 on CD4 T-cells in ALI/ARDS as compared to CG: 30 versus 7%, respectively (p = 0.063). In further characterizing T-cell subsets expressing high levels of CD152, we found the presence of IL-17 secreting CD4 T-cells in ALI/ARDS. In humans, ALI/ARDS due to infection is associated with a high level of T-cell activation and proliferation, along with the presence of Th17 cells, which are known to attract polymorphonuclears. Springer-Verlag Berlin Heidelberg, 2015 Risso K. Infectiologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut Kumar G. Infectiologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut Ticchioni M. Immunologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut Sanfiorenzo C. Pneumologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut Dellamonica J. Réanimation Médicale, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut Guillouet-de Salvador F. Infectiologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut Bernardin G. Réanimation Médicale, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut Marquette C.-H Pneumologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut Roger P.-M Infectiologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/6(2015-06-01), 1111-1118 0934-9723 34:6<1111 2015 34 10096 https://doi.org/10.1007/s10096-015-2333-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2333-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Risso K. Infectiologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut NATIONALLICENCE 700 1- Kumar G. Infectiologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut NATIONALLICENCE 700 1- Ticchioni M. Immunologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut NATIONALLICENCE 700 1- Sanfiorenzo C. Pneumologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut NATIONALLICENCE 700 1- Dellamonica J. Réanimation Médicale, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut NATIONALLICENCE 700 1- Guillouet-de Salvador F. Infectiologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut NATIONALLICENCE 700 1- Bernardin G. Réanimation Médicale, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut NATIONALLICENCE 700 1- Marquette C.-H Pneumologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut NATIONALLICENCE 700 1- Roger P.-M Infectiologie, Centre Hospitalier Universitaire de Nice, Université de Nice Sophia Antipolis, Nice, France aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/6(2015-06-01), 1111-1118 0934-9723 34:6<1111 2015 34 10096