caa a22 4500 606155651 CHVBK 20210128100604.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10096-015-2325-x doi (NATIONALLICENCE)springer-10.1007/s10096-015-2325-x Genetic variants of dental plaque Methanobrevibacter oralis [Elektronische Daten] [H. Huynh, V. Nkamga, M. Drancourt, G. Aboudharam] Methanobrevibacter oralis is the major methanogenic archaea found in the oral cavity. It has been implicated in periodontitis, including the severe form. It is unknown whether certain M. oralis genetic variants are associated with severe periodontitis. Here, we developed multispacer sequence typing (MST) as a sequencing-based genotyping method for the assessment of M. oralis. The sequencing of four intergenic spacers from a collection of 17 dental plaque M. oralis isolates obtained from seven individuals revealed 482 genetic polymorphisms, including 401 single nucleotide polymorphisms (83.2%), 55 deletions (11.4%) and 26 insertions (5.4%). Concatenation of the four spacers yielded nine genotypes, which were clustered into six groups with an index of discrimination of 0.919. One periodontitis patient may have harboured up to three genetic variants of M. oralis, revealing the previously unknown diversity of this archaea. MST will allow for the study of the dynamics of M. oralis populations, including inter-individual transmission and any correlations with the severity of periodontitis. Springer-Verlag Berlin Heidelberg, 2015 Huynh H. Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine, 27, Boulevard Jean Moulin, Marseille Cedex 5, France aut Nkamga V. Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine, 27, Boulevard Jean Moulin, Marseille Cedex 5, France aut Drancourt M. Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine, 27, Boulevard Jean Moulin, Marseille Cedex 5, France aut Aboudharam G. Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine, 27, Boulevard Jean Moulin, Marseille Cedex 5, France aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/6(2015-06-01), 1097-1101 0934-9723 34:6<1097 2015 34 10096 https://doi.org/10.1007/s10096-015-2325-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2325-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Huynh H. Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine, 27, Boulevard Jean Moulin, Marseille Cedex 5, France aut NATIONALLICENCE 700 1- Nkamga V. Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine, 27, Boulevard Jean Moulin, Marseille Cedex 5, France aut NATIONALLICENCE 700 1- Drancourt M. Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine, 27, Boulevard Jean Moulin, Marseille Cedex 5, France aut NATIONALLICENCE 700 1- Aboudharam G. Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR CNRS 7278, IRD 198, INSERM 1095, Faculté de Médecine, 27, Boulevard Jean Moulin, Marseille Cedex 5, France aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/6(2015-06-01), 1097-1101 0934-9723 34:6<1097 2015 34 10096