caa a22 4500 606155686 CHVBK 20210128100604.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10096-015-2431-9 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2431-9 Evaluation of the R-Biopharm RIDA®GENE Panton-Valentine leukocidin (PVL) kit for the detection of Staphylococcus aureus PVL from pus samples [Elektronische Daten] [C. Bouchiat, M. Bes, C. Bouveyron, F. Vandenesch, A. Tristan] Staphylococcus aureus Panton-Valentine leukocidin (PVL) is associated with primary skin and soft-tissue infections (SSTI). We aimed to divert the RIDA®GENE PVL kit (RBiopharm) from its intended use on cultures to the detection of PVL-encoding genes directly from pus samples. Performance was compared with that of the in-house PCR method developed by the French National Reference Centre for Staphylococci. From June 2013 to May 2014, pus samples from S. aureus SSTI were tested. Our in-house PCR was performed on parallel cultures as the gold standard, while the RIDA®GENE PVL assay was used directly on pus samples from the sterile container, or a swab or an Eswab previously dipped in the pus. The kit specificity was also evaluated with pus samples that grew Streptococcus pyogenes. S. aureus reference strains harboring PVL-encoding genes, including known polymorphisms, were also tested. A total of 56 S. aureus-containing pus samples (28 PVL + and 28 PVL−) were collected and analyzed. Sensitivity and specificity of the commercial kit were 96.4% and 100% respectively, with equal performance whether tested directly from the sterile container or the Eswab. Sensitivity was lower (67.9%) when the test was performed from a regular SSTI swab. None of the Streptococcus pyogenes pus samples scored positive (n = 5). Specificity was assessed using reference strains (n = 14); in all strains the PVL gene was correctly detected. This study identified the RIDA®GENE PVL kit as an efficient, sensitive, and specific tool for the rapid detection of PVL-encoding genes in pus samples. Springer-Verlag Berlin Heidelberg, 2015 Bouchiat C. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France aut Bes M. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France aut Bouveyron C. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France aut Vandenesch F. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France aut Tristan A. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/9(2015-09-01), 1905-1908 0934-9723 34:9<1905 2015 34 10096 https://doi.org/10.1007/s10096-015-2431-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2431-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bouchiat C. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France aut NATIONALLICENCE 700 1- Bes M. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France aut NATIONALLICENCE 700 1- Bouveyron C. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France aut NATIONALLICENCE 700 1- Vandenesch F. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France aut NATIONALLICENCE 700 1- Tristan A. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, Lyon, France aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/9(2015-09-01), 1905-1908 0934-9723 34:9<1905 2015 34 10096