caa a22 4500 606155724 CHVBK 20210128100605.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10096-015-2428-4 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2428-4 Direct molecular versus culture-based assessment of Gram-positive cocci in biopsies of patients with major abscesses and diabetic foot infections [Elektronische Daten] [M. Stappers, F. Hagen, P. Reimnitz, J. Mouton, J. Meis, I. Gyssens] Major abscesses and diabetic foot infections (DFIs) are predominant subtypes of complicated skin and skin structure infections (cSSSIs), and are mainly caused by Staphylococcus aureus and β-hemolytic streptococci. This study evaluates the potential benefit of direct pathogen-specific real-time polymerase chain reaction (PCR) assays in the identification of causative organisms of cSSSIs. One-hundred and fifty major abscess and 128 DFI biopsy samples were collected and microbial DNA was extracted by using the Universal Microbe Detection kit for tissue samples. Pathogen-specific PCRs were developed for S. aureus and its virulence factor Panton-Valentine leukocidin (PVL), Streptococcus pyogenes, S. agalactiae, S. dysgalactiae, and the S. anginosus group. Identification by pathogen-specific PCRs was compared to routine culture and both methods were considered as the gold standard for determination of the sensitivity and specificity of each assay. Direct real-time PCR assays of biopsy samples resulted in a 34% higher detection of S. aureus, 37% higher detection of S. pyogenes, 18% higher detection of S. agalactiae, 4% higher detection of S. dysgalactiae subspecies equisimilis, and 7% higher detection of the S. anginosus group, compared to routine bacterial culture. The presence of PVL was mainly confined to S. aureus isolated from major abscess but not DFI biopsy samples. In conclusion, our pathogen-specific real-time PCR assays had a higher yield than culture methods and could be an additional method for the detection of relevant causative pathogens in biopsies. The Author(s), 2015 Stappers M. Department of Internal Medicine, Radboud University Medical Center, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut Hagen F. Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands aut Reimnitz P. Bayer Healthcare Pharmaceuticals, Wuppertal, Germany aut Mouton J. Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands aut Meis J. Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands aut Gyssens I. Department of Internal Medicine, Radboud University Medical Center, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/9(2015-09-01), 1885-1892 0934-9723 34:9<1885 2015 34 10096 https://doi.org/10.1007/s10096-015-2428-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2428-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stappers M. Department of Internal Medicine, Radboud University Medical Center, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Hagen F. Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Reimnitz P. Bayer Healthcare Pharmaceuticals, Wuppertal, Germany aut NATIONALLICENCE 700 1- Mouton J. Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Meis J. Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Gyssens I. Department of Internal Medicine, Radboud University Medical Center, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/9(2015-09-01), 1885-1892 0934-9723 34:9<1885 2015 34 10096