caa a22 4500 606155813 CHVBK 20210128100605.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10096-015-2416-8 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2416-8 Prolonging culture to 15 days improves bacterial detection in bone and joint infections [Elektronische Daten] [L. Drago, E. De Vecchi, L. Cappelletti, C. Vassena, M. Toscano, M. Bortolin, R. Mattina, C. Romanò] Since the optimal incubation period of cultures for diagnosis of bone and joint infections is still a matter of debate, the present study aimed to evaluate the effects of different incubation periods (5 and 15days) on microbial isolation. Samples from 387 patients with bone and joint infections (including prosthetic ones) were analyzed from March 2012 to February 2014. In 197 patients (51%) growth was obtained within 48hrs, while in 124 (32%) and 66 (17%) patients cultures yielded positive results within and after 5days of incubation, respectively. Of 449 microorganisms isolated, 247 grew within 48hrs, 131 within the first 5days of incubation while 71 were isolated after 5 days. Staphylococcus aureus was the most frequently isolated pathogen within 48hrs, while Propionibacteria were prevalently isolated after 5days of incubation. Interestingly, about 25% of microorganisms isolated after 5days of incubation were coagulase-negative staphylococci. Extending incubation period of broth cultures improves isolation rates of pathogens involved in bone and joint infections thus improving management of these infections. Springer-Verlag Berlin Heidelberg, 2015 Drago L. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy aut De Vecchi E. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy aut Cappelletti L. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy aut Vassena C. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy aut Toscano M. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy aut Bortolin M. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy aut Mattina R. Department of Biomedical, Surgical and Odontoiatric Sciences, University of Milan, Via C. Pascal 36, 20133, Milan, Italy aut Romanò C. Centre for Reconstructive Surgery and Bone Infections, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/9(2015-09-01), 1809-1813 0934-9723 34:9<1809 2015 34 10096 https://doi.org/10.1007/s10096-015-2416-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2416-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Drago L. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy aut NATIONALLICENCE 700 1- De Vecchi E. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy aut NATIONALLICENCE 700 1- Cappelletti L. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy aut NATIONALLICENCE 700 1- Vassena C. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy aut NATIONALLICENCE 700 1- Toscano M. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy aut NATIONALLICENCE 700 1- Bortolin M. Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy aut NATIONALLICENCE 700 1- Mattina R. Department of Biomedical, Surgical and Odontoiatric Sciences, University of Milan, Via C. Pascal 36, 20133, Milan, Italy aut NATIONALLICENCE 700 1- Romanò C. Centre for Reconstructive Surgery and Bone Infections, IRCCS Galeazzi Orthopaedic Institute, Via R. Galeazzi 4, 20161, Milan, Italy aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/9(2015-09-01), 1809-1813 0934-9723 34:9<1809 2015 34 10096