caa a22 4500 606155821 CHVBK 20210128100605.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10096-015-2410-1 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2410-1 Fusarium spp. is able to grow and invade healthy human nails as a single source of nutrients [Elektronische Daten] [J. Galletti, M. Negri, F. Grassi, É. Kioshima-Cotica, T. Svidzinski] Onychomycosis caused by Fusarium spp. is emerging, but some factors associated with its development remain unclear, such as whether this genus is keratinolytic. The main aim of the present study was to evaluate the ability of Fusarium to use the human nail as a single source of nutrients. We also performed an epidemiological study and antifungal susceptibility testing of Fusarium spp. that were isolated from patients with onychomycosis. The epidemiological study showed that Fusarium species accounted for 12.4% of onychomycosis cases, and it was the most common among nondermatophyte molds. The most frequent species identified were F. oxysporum (36.5%), F. solani (31.8%), and F. subglutinans (8.3%). Fluconazole was not active against Fusarium spp., and the response to terbinafine varied according to species. Fusarium was able to grow in vitro without the addition of nutrients and invade healthy nails. Thus, we found that Fusarium uses keratin as a single source of nutrients, and the model proposed herein may be useful for future studies on the pathogenesis of onychomycosis. Springer-Verlag Berlin Heidelberg, 2015 Galletti J. Medical Mycology Laboratory, Departamento de Análises Clínicas e Biomedicina da Universidade Estadual de Maringá, Maringá, PR, Brazil aut Negri M. Medical Mycology Laboratory, Departamento de Análises Clínicas e Biomedicina da Universidade Estadual de Maringá, Maringá, PR, Brazil aut Grassi F. Department of Design and Fashion, Universidade Estadual de Maringá, Maringá, PR, Brazil aut Kioshima-Cotica É. Medical Mycology Laboratory, Departamento de Análises Clínicas e Biomedicina da Universidade Estadual de Maringá, Maringá, PR, Brazil aut Svidzinski T. Medical Mycology Laboratory, Departamento de Análises Clínicas e Biomedicina da Universidade Estadual de Maringá, Maringá, PR, Brazil aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/9(2015-09-01), 1767-1772 0934-9723 34:9<1767 2015 34 10096 https://doi.org/10.1007/s10096-015-2410-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2410-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Galletti J. Medical Mycology Laboratory, Departamento de Análises Clínicas e Biomedicina da Universidade Estadual de Maringá, Maringá, PR, Brazil aut NATIONALLICENCE 700 1- Negri M. Medical Mycology Laboratory, Departamento de Análises Clínicas e Biomedicina da Universidade Estadual de Maringá, Maringá, PR, Brazil aut NATIONALLICENCE 700 1- Grassi F. Department of Design and Fashion, Universidade Estadual de Maringá, Maringá, PR, Brazil aut NATIONALLICENCE 700 1- Kioshima-Cotica É. Medical Mycology Laboratory, Departamento de Análises Clínicas e Biomedicina da Universidade Estadual de Maringá, Maringá, PR, Brazil aut NATIONALLICENCE 700 1- Svidzinski T. Medical Mycology Laboratory, Departamento de Análises Clínicas e Biomedicina da Universidade Estadual de Maringá, Maringá, PR, Brazil aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/9(2015-09-01), 1767-1772 0934-9723 34:9<1767 2015 34 10096