caa a22 4500 606155899 CHVBK 20210128100605.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10096-015-2415-9 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2415-9 Comparison of Sofia Legionella FIA and BinaxNOW® Legionella urinary antigen card in two national reference centers [Elektronische Daten] [L. Beraud, K. Gervasoni, A. Freydiere, G. Descours, A. Ranc, F. Vandenesch, G. Lina, V. Gaia, S. Jarraud] The Sofia Legionella Fluorescence Immunoassay (FIA; Quidel) is a recently introduced rapid immunochromatographic diagnostic test for Legionnaires' disease using immunofluorescence technology designed to enhance its sensitivity. The aim of this study was to evaluate its performance for the detection of urinary antigens for Legionella pneumophila serogroup 1 in two National Reference Centers for Legionella. The sensitivity and specificity of the Sofia Legionella FIA test were determined in concentrated and nonconcentrated urine samples, before and after boiling, in comparison with the BinaxNOW® Legionella Urinary Antigen Card (UAC; Alere). Compared with BinaxNOW® Legionella UAC, the sensitivity of the Sofia Legionella test was slightly higher in nonconcentrated urine samples and was identical in concentrated urine samples. The specificity of the Sofia Legionella FIA test was highly reduced by the concentration of urine samples. In nonconcentrated samples, a lack of specificity was observed in 2.3% of samples, all of them resolved by heat treatment. The Sofia Legionella FIA is a sensitive test for detecting Legionella urinary antigens with no previous urine concentration. However, all positive samples have to be re-tested after boiling to reach a high specificity. The reading is automatized on the Sofia analyzer, which can be connected to laboratory information systems, facilitating accurate and rapid reporting of results. The Author(s), 2015 Beraud L. French Reference Center for Legionella, Laboratory of Microbiology, Groupe Hospitalier Est, Hospices Civils de Lyon, Lyon, France aut Gervasoni K. Reference Center for Legionella, Laboratory of Microbiology, Department of Laboratory Medecine, Ente Ospedaliero Cantonale, Bellinzona, Switzerland aut Freydiere A. French Reference Center for Legionella, Laboratory of Microbiology, Groupe Hospitalier Est, Hospices Civils de Lyon, Lyon, France aut Descours G. French Reference Center for Legionella, Laboratory of Microbiology, Groupe Hospitalier Est, Hospices Civils de Lyon, Lyon, France aut Ranc A. French Reference Center for Legionella, Laboratory of Microbiology, Groupe Hospitalier Est, Hospices Civils de Lyon, Lyon, France aut Vandenesch F. French Reference Center for Legionella, Laboratory of Microbiology, Groupe Hospitalier Est, Hospices Civils de Lyon, Lyon, France aut Lina G. French Reference Center for Legionella, Laboratory of Microbiology, Groupe Hospitalier Est, Hospices Civils de Lyon, Lyon, France aut Gaia V. Reference Center for Legionella, Laboratory of Microbiology, Department of Laboratory Medecine, Ente Ospedaliero Cantonale, Bellinzona, Switzerland aut Jarraud S. French Reference Center for Legionella, Laboratory of Microbiology, Groupe Hospitalier Est, Hospices Civils de Lyon, Lyon, France aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/9(2015-09-01), 1803-1807 0934-9723 34:9<1803 2015 34 10096 https://doi.org/10.1007/s10096-015-2415-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2415-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Beraud L. French Reference Center for Legionella, Laboratory of Microbiology, Groupe Hospitalier Est, Hospices Civils de Lyon, Lyon, France aut NATIONALLICENCE 700 1- Gervasoni K. Reference Center for Legionella, Laboratory of Microbiology, Department of Laboratory Medecine, Ente Ospedaliero Cantonale, Bellinzona, Switzerland aut NATIONALLICENCE 700 1- Freydiere A. French Reference Center for Legionella, Laboratory of Microbiology, Groupe Hospitalier Est, Hospices Civils de Lyon, Lyon, France aut NATIONALLICENCE 700 1- Descours G. French Reference Center for Legionella, Laboratory of Microbiology, Groupe Hospitalier Est, Hospices Civils de Lyon, Lyon, France aut NATIONALLICENCE 700 1- Ranc A. French Reference Center for Legionella, Laboratory of Microbiology, Groupe Hospitalier Est, Hospices Civils de Lyon, Lyon, France aut NATIONALLICENCE 700 1- Vandenesch F. French Reference Center for Legionella, Laboratory of Microbiology, Groupe Hospitalier Est, Hospices Civils de Lyon, Lyon, France aut NATIONALLICENCE 700 1- Lina G. French Reference Center for Legionella, Laboratory of Microbiology, Groupe Hospitalier Est, Hospices Civils de Lyon, Lyon, France aut NATIONALLICENCE 700 1- Gaia V. Reference Center for Legionella, Laboratory of Microbiology, Department of Laboratory Medecine, Ente Ospedaliero Cantonale, Bellinzona, Switzerland aut NATIONALLICENCE 700 1- Jarraud S. French Reference Center for Legionella, Laboratory of Microbiology, Groupe Hospitalier Est, Hospices Civils de Lyon, Lyon, France aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/9(2015-09-01), 1803-1807 0934-9723 34:9<1803 2015 34 10096