caa a22 4500 606155910 CHVBK 20210128100606.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s10096-015-2413-y doi (NATIONALLICENCE)springer-10.1007/s10096-015-2413-y Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS [Elektronische Daten] [L. Veron, S. Mailler, V. Girard, B. Muller, G. L'Hostis, C. Ducruix, A. Lesenne, A. Richez, H. Rostaing, V. Lanet, S. Ghirardi, A. van Belkum, F. Mallard] Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) has been introduced in clinical routine microbiology laboratories. For the rapid diagnosis of urinary tract infections, culture-independent methods prior MALDI-mediated identification have been described. Here, we describe a comparison of three of these methods based on their performance of bacterial identification and their potential as a routine tool for microbiology labs : (i) differential centrifugation, (ii) urine filtration and (iii) a 5-h bacterial cultivation on solid culture media. For 19 urine samples, all methods were directly compared and correct bacterial species identification by MALDI was used as performance indicator. A higher percentage of correct MALDI identification was obtained after filtration (78.9%) and the growth-based method (84.2%) as compared to differential centrifugation (68.4%). Additional testing of 76 mono-microbial specimens (bacteriuria > 105CFU/mL) confirmed the good performance of short growth with a 90.8% correct MALDI score, with a potentially better fit to the routine workflow of microbiology labs. Springer-Verlag Berlin Heidelberg, 2015 Veron L. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l'Orme, 69280, Marcy L'Etoile, France aut Mailler S. Microbiology R&D, bioMérieux S.A., 38390, La Balme Les Grottes, France aut Girard V. Microbiology R&D, bioMérieux S.A., 38390, La Balme Les Grottes, France aut Muller B. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l'Orme, 69280, Marcy L'Etoile, France aut L'Hostis G. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l'Orme, 69280, Marcy L'Etoile, France aut Ducruix C. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l'Orme, 69280, Marcy L'Etoile, France aut Lesenne A. Novescia, Paris-Sud, 91320, Wissous, France aut Richez A. Medical Biology Laboratory, St-Luc/St-Joseph, Hospices Civils de Lyon, 69007, Lyon, France aut Rostaing H. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l'Orme, 69280, Marcy L'Etoile, France aut Lanet V. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l'Orme, 69280, Marcy L'Etoile, France aut Ghirardi S. Microbiology R&D, bioMérieux S.A., 38390, La Balme Les Grottes, France aut van Belkum A. Microbiology R&D, bioMérieux S.A., 38390, La Balme Les Grottes, France aut Mallard F. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l'Orme, 69280, Marcy L'Etoile, France aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/9(2015-09-01), 1787-1795 0934-9723 34:9<1787 2015 34 10096 https://doi.org/10.1007/s10096-015-2413-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2413-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Veron L. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l'Orme, 69280, Marcy L'Etoile, France aut NATIONALLICENCE 700 1- Mailler S. Microbiology R&D, bioMérieux S.A., 38390, La Balme Les Grottes, France aut NATIONALLICENCE 700 1- Girard V. Microbiology R&D, bioMérieux S.A., 38390, La Balme Les Grottes, France aut NATIONALLICENCE 700 1- Muller B. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l'Orme, 69280, Marcy L'Etoile, France aut NATIONALLICENCE 700 1- L'Hostis G. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l'Orme, 69280, Marcy L'Etoile, France aut NATIONALLICENCE 700 1- Ducruix C. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l'Orme, 69280, Marcy L'Etoile, France aut NATIONALLICENCE 700 1- Lesenne A. Novescia, Paris-Sud, 91320, Wissous, France aut NATIONALLICENCE 700 1- Richez A. Medical Biology Laboratory, St-Luc/St-Joseph, Hospices Civils de Lyon, 69007, Lyon, France aut NATIONALLICENCE 700 1- Rostaing H. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l'Orme, 69280, Marcy L'Etoile, France aut NATIONALLICENCE 700 1- Lanet V. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l'Orme, 69280, Marcy L'Etoile, France aut NATIONALLICENCE 700 1- Ghirardi S. Microbiology R&D, bioMérieux S.A., 38390, La Balme Les Grottes, France aut NATIONALLICENCE 700 1- van Belkum A. Microbiology R&D, bioMérieux S.A., 38390, La Balme Les Grottes, France aut NATIONALLICENCE 700 1- Mallard F. Innovation/Technology Research Department, bioMérieux S.A., Chemin de l'Orme, 69280, Marcy L'Etoile, France aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/9(2015-09-01), 1787-1795 0934-9723 34:9<1787 2015 34 10096