caa a22 4500 60615597X CHVBK 20210128100606.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10096-015-2359-0 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2359-0 Recovery of dengue virus from urine samples by real-time RT-PCR [Elektronische Daten] [D. Van den Bossche, L. Cnops, M. Van Esbroeck] Recently, reverse transcription polymerase chain reaction (RT-PCR) for dengue virus (DENV) has been reported to test positive in urine samples for a longer time frame than in serum. We evaluated two RNA extraction procedures from urine and investigated the stability of DENV RNA in urine and serum up to 1year at different storage temperatures. In addition, 24 urine samples collected from patients with a recent infection were tested with DENV real-time RT-PCR and compared to the RT-PCR results on serum. Five patients with an acute DENV infection were followed up for 6months by RT-PCR on urine. The automated extraction method with the MagNA Pure LC 2.0 device had a higher yield of DENV RNA compared to the manual QIAGEN method, explained by the higher volume used in the former method. DENV RNA in both serum and urine was stable at room temperature up to 1month and at 4°C and −20°C for at least 1year. The detection rate by RT-PCR on urine was 50% (4/8) until day 7, 100% (6/6) between 1 and 3weeks after symptom onset, and 25% (2/8) thereafter. Generally, DENV RNA concentrations are higher in serum than in urine up till day 7, switching to lower concentrations in serum thereafter. Peak concentrations in urine are reached around day 10, and RNA becomes undetectable 3 to 4weeks following disease onset. This diagnostic tool is of added value in clinical settings by extending the period during which DENV infections are diagnosed by RT-PCR. Springer-Verlag Berlin Heidelberg, 2015 Van den Bossche D. Central Laboratory of Clinical Biology, Department of Clinical Sciences, Institute of Tropical Medicine (ITM), Kronenburgstraat 43/3, 2000, Antwerp, Belgium aut Cnops L. Central Laboratory of Clinical Biology, Department of Clinical Sciences, Institute of Tropical Medicine (ITM), Kronenburgstraat 43/3, 2000, Antwerp, Belgium aut Van Esbroeck M. Central Laboratory of Clinical Biology, Department of Clinical Sciences, Institute of Tropical Medicine (ITM), Kronenburgstraat 43/3, 2000, Antwerp, Belgium aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/7(2015-07-01), 1361-1367 0934-9723 34:7<1361 2015 34 10096 https://doi.org/10.1007/s10096-015-2359-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2359-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Van den Bossche D. Central Laboratory of Clinical Biology, Department of Clinical Sciences, Institute of Tropical Medicine (ITM), Kronenburgstraat 43/3, 2000, Antwerp, Belgium aut NATIONALLICENCE 700 1- Cnops L. Central Laboratory of Clinical Biology, Department of Clinical Sciences, Institute of Tropical Medicine (ITM), Kronenburgstraat 43/3, 2000, Antwerp, Belgium aut NATIONALLICENCE 700 1- Van Esbroeck M. Central Laboratory of Clinical Biology, Department of Clinical Sciences, Institute of Tropical Medicine (ITM), Kronenburgstraat 43/3, 2000, Antwerp, Belgium aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/7(2015-07-01), 1361-1367 0934-9723 34:7<1361 2015 34 10096