caa a22 4500 60615602X CHVBK 20210128100606.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10096-015-2355-4 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2355-4 Study of the diversity and short-chain fatty acids production by the bacterial community in overweight and obese Mexican children [Elektronische Daten] [S. Murugesan, M. Ulloa-Martínez, H. Martínez-Rojano, F. Galván-Rodríguez, C. Miranda-Brito, M. Romano, A. Piña-Escobedo, M. Pizano-Zárate, C. Hoyo-Vadillo, J. García-Mena] Obesity and overweight are health problems of multifactorial etiology, which may include changes in the microbiome. In Mexico, more than 30% of the child population between 5 and 11years of age suffer from being overweight or are obese, which makes it a public health issue in progress. The purpose of this work was to measure the short-chain fatty acid concentration by high-performance liquid chromatography (HPLC), and to characterize the bacterial diversity by ion torrent semiconductor sequencing, of 16S rDNA libraries prepared from stools collected from a sample of well-characterized Mexican children for normal weight, overweight, and obese conditions by anthropometric and biochemical criteria. We found that triglyceride levels are increased in overweight and obese children, who presented altered propionic and butyric acid concentrations in feces. In addition, although the colon microbiota did not show a clear bacterial dysbiosis among the three conditions, the abundance of some particular bacteria was changed with respect to normal controls. We conclude from our results that the imbalance in the abundance of at least nine different bacteria as well as altered short-chain fatty acid concentration in feces is associated to the overweight and obese conditions of Mexican children. Springer-Verlag Berlin Heidelberg, 2015 Murugesan S. Departamento de Genética y Biología Molecular, Cinvestav-IPN Unidad Zacatenco, 07360, México, DF, Mexico aut Ulloa-Martínez M. Departamento de Genética y Biología Molecular, Cinvestav-IPN Unidad Zacatenco, 07360, México, DF, Mexico aut Martínez-Rojano H. Escuela Superior de Medicina del Instituto Politécnico Nacional, México, DF, Mexico aut Galván-Rodríguez F. Departamento de Farmacología, Cinvestav-IPN, México, DF, Mexico aut Miranda-Brito C. Departamento de Fisiología Biofísica y Neurociencias, Cinvestav-IPN, México, DF, Mexico aut Romano M. Departamento de Fisiología Biofísica y Neurociencias, Cinvestav-IPN, México, DF, Mexico aut Piña-Escobedo A. Departamento de Genética y Biología Molecular, Cinvestav-IPN Unidad Zacatenco, 07360, México, DF, Mexico aut Pizano-Zárate M. Departamento de Nutrición, Instituto Nacional de Perinatología, México, DF, Mexico aut Hoyo-Vadillo C. Departamento de Farmacología, Cinvestav-IPN, México, DF, Mexico aut García-Mena J. Departamento de Genética y Biología Molecular, Cinvestav-IPN Unidad Zacatenco, 07360, México, DF, Mexico aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/7(2015-07-01), 1337-1346 0934-9723 34:7<1337 2015 34 10096 https://doi.org/10.1007/s10096-015-2355-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2355-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Murugesan S. Departamento de Genética y Biología Molecular, Cinvestav-IPN Unidad Zacatenco, 07360, México, DF, Mexico aut NATIONALLICENCE 700 1- Ulloa-Martínez M. Departamento de Genética y Biología Molecular, Cinvestav-IPN Unidad Zacatenco, 07360, México, DF, Mexico aut NATIONALLICENCE 700 1- Martínez-Rojano H. Escuela Superior de Medicina del Instituto Politécnico Nacional, México, DF, Mexico aut NATIONALLICENCE 700 1- Galván-Rodríguez F. Departamento de Farmacología, Cinvestav-IPN, México, DF, Mexico aut NATIONALLICENCE 700 1- Miranda-Brito C. Departamento de Fisiología Biofísica y Neurociencias, Cinvestav-IPN, México, DF, Mexico aut NATIONALLICENCE 700 1- Romano M. Departamento de Fisiología Biofísica y Neurociencias, Cinvestav-IPN, México, DF, Mexico aut NATIONALLICENCE 700 1- Piña-Escobedo A. Departamento de Genética y Biología Molecular, Cinvestav-IPN Unidad Zacatenco, 07360, México, DF, Mexico aut NATIONALLICENCE 700 1- Pizano-Zárate M. Departamento de Nutrición, Instituto Nacional de Perinatología, México, DF, Mexico aut NATIONALLICENCE 700 1- Hoyo-Vadillo C. Departamento de Farmacología, Cinvestav-IPN, México, DF, Mexico aut NATIONALLICENCE 700 1- García-Mena J. Departamento de Genética y Biología Molecular, Cinvestav-IPN Unidad Zacatenco, 07360, México, DF, Mexico aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/7(2015-07-01), 1337-1346 0934-9723 34:7<1337 2015 34 10096