caa a22 4500 606156089 CHVBK 20210128100606.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10096-015-2371-4 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2371-4 Impacts of enterotoxin gene cluster-encoded superantigens on local and systemic experimental Staphylococcus aureus infections [Elektronische Daten] [F. Nowrouzian, A. Ali, C. Badiou, O. Dauwalder, G. Lina, E. Josefsson] Staphylococcus aureus is both a component of the normal skin flora and an important pathogen. It expresses a range of recognized and putative virulence factors, such as enterotoxins with superantigenic properties. Several superantigen genes, i.e., seg, sei, selm, seln, and selo, are encoded by the enterotoxin gene cluster (egc), which is found in the majority of S. aureus isolates. Carriage of egc is associated with fitness of S. aureus in the gut microbiota, but it is not known if it contributes to pathogenicity. We constructed egc+ (functional for the seg, selm, and selo genes) and isogenic egc− S. aureus mutants, and investigated their virulence profiles in murine infection models. No effect of egc was seen in a local skin and soft tissue infection model, but in an invasive infection model, increased weight loss was observed after infection with the egc+ as compared to the egc− mutant. Mortality and arthritis were not affected by egc status. Our data suggest that egc has limited effects on the virulence of S. aureus. It may primarily function as a colonization factor increasing commensal fitness, although it might have some aggravating effects on the infection when the bacteria reach the blood. Springer-Verlag Berlin Heidelberg, 2015 Nowrouzian F. Department of Infectious Diseases, Institute of Biomedicine, University of Gothenburg, Guldhedsgatan 10, 413 46, Göteborg, Sweden aut Ali A. Department of Rheumatology and Inflammation Research, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden aut Badiou C. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, CIRI, Université Lyon 1, Inserm U1111, CNRS UMR5308, Ecole Normale Supérieure de Lyon, Lyon, France aut Dauwalder O. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, CIRI, Université Lyon 1, Inserm U1111, CNRS UMR5308, Ecole Normale Supérieure de Lyon, Lyon, France aut Lina G. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, CIRI, Université Lyon 1, Inserm U1111, CNRS UMR5308, Ecole Normale Supérieure de Lyon, Lyon, France aut Josefsson E. Department of Rheumatology and Inflammation Research, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/7(2015-07-01), 1443-1449 0934-9723 34:7<1443 2015 34 10096 https://doi.org/10.1007/s10096-015-2371-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2371-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nowrouzian F. Department of Infectious Diseases, Institute of Biomedicine, University of Gothenburg, Guldhedsgatan 10, 413 46, Göteborg, Sweden aut NATIONALLICENCE 700 1- Ali A. Department of Rheumatology and Inflammation Research, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden aut NATIONALLICENCE 700 1- Badiou C. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, CIRI, Université Lyon 1, Inserm U1111, CNRS UMR5308, Ecole Normale Supérieure de Lyon, Lyon, France aut NATIONALLICENCE 700 1- Dauwalder O. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, CIRI, Université Lyon 1, Inserm U1111, CNRS UMR5308, Ecole Normale Supérieure de Lyon, Lyon, France aut NATIONALLICENCE 700 1- Lina G. Centre National de Référence des Staphylocoques, Hospices Civils de Lyon, CIRI, Université Lyon 1, Inserm U1111, CNRS UMR5308, Ecole Normale Supérieure de Lyon, Lyon, France aut NATIONALLICENCE 700 1- Josefsson E. Department of Rheumatology and Inflammation Research, Institute of Medicine, University of Gothenburg, Gothenburg, Sweden aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/7(2015-07-01), 1443-1449 0934-9723 34:7<1443 2015 34 10096