caa a22 4500 606156135 CHVBK 20210128100607.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10096-015-2365-2 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2365-2 Antimicrobial susceptibility of rapidly growing mycobacteria using the rapid colorimetric method [Elektronische Daten] [I. Ramis, M. Cnockaert, A. von Groll, C. Nogueira, S. Leão, E. Andre, A. Simon, J. Palomino, P. da Silva, P. Vandamme, A. Martin] Drug susceptibility testing (DST) of rapidly growing mycobacteria (RGM) are recommended for guiding the antimicrobial therapy. We have evaluated the use of resazurin in Mueller-Hinton medium (MHR) for MIC determination of RGM and compared the results with those obtained with the reference standard broth microdilution in Mueller-Hinton (MH) and with the resazurin microtiter assay (REMA) in 7H9 broth. The MIC of eight drugs: amikacin (AMI), cefoxitin (FOX), ciprofloxacin (CIP), clarithromycin (CLA), doxycycline (DOX), linezolid (LZD), moxifloxacin (MXF) and trimethoprim-sulfamethoxazole (TMP-SMX) were evaluated against 76 RGM (18 species) using three methods (MH, MHR, and REMA) in a 96-well plate format incubated at 37°C over 3-5days. Results obtained in the MH plates were interpreted by the appearance of turbidity at the bottom of the well before adding the resazurin. MHR and 7H9-REMA plates were read by visual observation for a change in color from blue to pink. The majority of results were obtained at day 5 for MH and 1day after for MHR and 7H9-REMA. However, the preliminary experiment on time to positivity results using the reference strain showed that the resazurin can be added to the MH at day 2 to produce the results at day 3, but future studies with large sets of strains are required to confirm this suggestion. A high level of agreement (kappa 1.000-0.884) was obtained between the MH and the MHR. Comparison of results obtained with 7H9-REMA, on the other hand, revealed several discrepancies and a lower level of agreement (kappa 1.000-0.111). The majority of the strains were resistant to DOX and TMP-SMX, and the most active antimicrobials for RGM were AMI and FOX. In the present study, MHR represented an excellent alternative for MIC determination of RGM. The results could be read reliably, more easily, and more quickly than with the classical MH method. Springer-Verlag Berlin Heidelberg, 2015 Ramis I. Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Pelotas, Brazil aut Cnockaert M. Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium aut von Groll A. Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil aut Nogueira C. Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil aut Leão S. Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil aut Andre E. Pôle de Microbiologie, Institut de Recherche Expérimentale et Clinique, UC Louvain, Brussels, Belgium aut Simon A. Pôle de Microbiologie, Institut de Recherche Expérimentale et Clinique, UC Louvain, Brussels, Belgium aut Palomino J. Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium aut da Silva P. Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Pelotas, Brazil aut Vandamme P. Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium aut Martin A. Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/7(2015-07-01), 1403-1413 0934-9723 34:7<1403 2015 34 10096 https://doi.org/10.1007/s10096-015-2365-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2365-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ramis I. Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Pelotas, Brazil aut NATIONALLICENCE 700 1- Cnockaert M. Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium aut NATIONALLICENCE 700 1- von Groll A. Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Nogueira C. Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Leão S. Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Andre E. Pôle de Microbiologie, Institut de Recherche Expérimentale et Clinique, UC Louvain, Brussels, Belgium aut NATIONALLICENCE 700 1- Simon A. Pôle de Microbiologie, Institut de Recherche Expérimentale et Clinique, UC Louvain, Brussels, Belgium aut NATIONALLICENCE 700 1- Palomino J. Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium aut NATIONALLICENCE 700 1- da Silva P. Centro de Desenvolvimento Tecnológico (CDTec), Universidade Federal de Pelotas, Pelotas, Brazil aut NATIONALLICENCE 700 1- Vandamme P. Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium aut NATIONALLICENCE 700 1- Martin A. Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/7(2015-07-01), 1403-1413 0934-9723 34:7<1403 2015 34 10096