caa a22 4500 606156143 CHVBK 20210128100607.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10096-015-2343-8 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2343-8 Preparation and evaluation of monoclonal antibodies against chlamydial protease-like activity factor to detect Chlamydia pneumoniae antigen in early pediatric pneumonia [Elektronische Daten] [J. Zheng, T. Ding, Z. Chen, H. Fang, H. Li, H. Lu, Y. Wu] Chlamydia pneumoniae causes diseases in humans, including community-acquired pneumonia, bronchitis, and sinusitis. It is also associated with atherosclerosis, coronary heart disease, and hyperlipidemia. In this study, we investigated novel materials with which to develop a sensitive and specific method to identify early C. pneumoniae infection, to allow more effective clinical treatment and prevention. We prepared novel monoclonal antibodies (mAbs) against a recombinant protein equivalent to the immunodominant region of chlamydial protease-like activity factor (CPAF) from C. pneumoniae. The mAbs specifically reacted with the endogenous CPAF antigen of the C. pneumoniae type strain in immunoblotting and indirect immunofluorescence (IIF) assays, but did not react with C. trachomatis type strains or genital secretions from patients with acute C. trachomatis infection. The mAb with the highest titer was used to develop a new IIF assay and enzyme-linked immunosorbent assay (ELISA) to detect the C. pneumoniae antigen in clinical specimens from child patients suspected of pneumonia. The sensitivity, specificity, and concordance rate of the mAb-based IIF and ELISA tests were compared with those of polymerase chain reaction (PCR). Our results show that these mAbs have excellent specificity and may be used to develop new screening tools for the diagnosis of early pediatric pneumonia. Springer-Verlag Berlin Heidelberg, 2015 Zheng J. Shanghai Public Health Clinical Center, Fudan University, Shanghai, China aut Ding T. Department of Urology, Yuebei People's Hospital Affiliated to Shantou University Medical College, Shaoguan, China aut Chen Z. Shanghai Public Health Clinical Center, Fudan University, Shanghai, China aut Fang H. Shanghai Public Health Clinical Center, Fudan University, Shanghai, China aut Li H. Shanghai Public Health Clinical Center, Fudan University, Shanghai, China aut Lu H. Shanghai Public Health Clinical Center, Fudan University, Shanghai, China aut Wu Y. Institute of Pathogenic Biology, Medical College, University of South China, 28 West Changsheng Road, 421009, Hengyang, China aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/7(2015-07-01), 1319-1326 0934-9723 34:7<1319 2015 34 10096 https://doi.org/10.1007/s10096-015-2343-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2343-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zheng J. Shanghai Public Health Clinical Center, Fudan University, Shanghai, China aut NATIONALLICENCE 700 1- Ding T. Department of Urology, Yuebei People's Hospital Affiliated to Shantou University Medical College, Shaoguan, China aut NATIONALLICENCE 700 1- Chen Z. Shanghai Public Health Clinical Center, Fudan University, Shanghai, China aut NATIONALLICENCE 700 1- Fang H. Shanghai Public Health Clinical Center, Fudan University, Shanghai, China aut NATIONALLICENCE 700 1- Li H. Shanghai Public Health Clinical Center, Fudan University, Shanghai, China aut NATIONALLICENCE 700 1- Lu H. Shanghai Public Health Clinical Center, Fudan University, Shanghai, China aut NATIONALLICENCE 700 1- Wu Y. Institute of Pathogenic Biology, Medical College, University of South China, 28 West Changsheng Road, 421009, Hengyang, China aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/7(2015-07-01), 1319-1326 0934-9723 34:7<1319 2015 34 10096