caa a22 4500 60615616X CHVBK 20210128100607.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10096-015-2366-1 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2366-1 Long-term persistence of a multi-resistant methicillin-susceptible Staphylococcus aureus (MR-MSSA) clone at a university hospital in southeast Sweden, without further transmission within the region [Elektronische Daten] [M. Lindqvist, B. Isaksson, J. Swanberg, R. Skov, A. Larsen, J. Larsen, A. Petersen, A. Hällgren] The objective of this study was to characterise isolates of methicillin-susceptible Staphylococcus aureus (MSSA) with resistance to clindamycin and/or tobramycin in southeast Sweden, including the previously described ECT-R clone (t002) found in Östergötland County, focusing on clonal relatedness, virulence determinants and existence of staphylococcal cassette chromosome (SCC) mec remnants. MSSA isolates with resistance to clindamycin and/or tobramycin were collected from the three county councils in southeast Sweden and investigated with spa typing, polymerase chain reaction (PCR) targeting the SCCmec right extremity junction (MREJ) and DNA microarray technology. The 98 isolates were divided into 40 spa types, and by microarray clustered in 17 multi-locus sequence typing (MLST) clonal complexes (MLST-CCs). All isolates with combined resistance to clindamycin and tobramycin (n = 12) from Östergötland County and two additional isolates (clindamycin-R) were designated as spa type t002, MREJ type ii and were clustered in CC5, together with a representative isolate of the ECT-R clone, indicating the clone's persistence. These isolates also carried several genes encoding exotoxins, Q9XB68-dcs and qacC. Of the isolates in CC15, 83% (25/30) were tobramycin-resistant and were designated spa type t084. Of these, 68% (17/25) were isolated from new-borns in all three counties. The persistence of the ECT-R clone in Östergötland County, although not found in any other county in the region, carrying certain virulence factors that possibly enhance its survival in the hospital environment, highlights the fact that basic hygiene guidelines must be maintained even when MRSA prevalence is low. Springer-Verlag Berlin Heidelberg, 2015 Lindqvist M. Department of Infection Control, County Council of Östergötland, Linköping, Sweden aut Isaksson B. Department of Infection Control, County Council of Östergötland, Linköping, Sweden aut Swanberg J. Clinical Microbiology Laboratory, Ryhov Hospital, Jönköping, Sweden aut Skov R. Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark aut Larsen A. Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark aut Larsen J. Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark aut Petersen A. Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark aut Hällgren A. Division of Infectious Diseases, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/7(2015-07-01), 1415-1422 0934-9723 34:7<1415 2015 34 10096 https://doi.org/10.1007/s10096-015-2366-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2366-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lindqvist M. Department of Infection Control, County Council of Östergötland, Linköping, Sweden aut NATIONALLICENCE 700 1- Isaksson B. Department of Infection Control, County Council of Östergötland, Linköping, Sweden aut NATIONALLICENCE 700 1- Swanberg J. Clinical Microbiology Laboratory, Ryhov Hospital, Jönköping, Sweden aut NATIONALLICENCE 700 1- Skov R. Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Larsen A. Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Larsen J. Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Petersen A. Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Hällgren A. Division of Infectious Diseases, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/7(2015-07-01), 1415-1422 0934-9723 34:7<1415 2015 34 10096