caa a22 4500 606156178 CHVBK 20210128100607.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s10096-015-2376-z doi (NATIONALLICENCE)springer-10.1007/s10096-015-2376-z Evaluation of several phenotypic methods for the detection of carbapenemase-producing Pseudomonas aeruginosa [Elektronische Daten] [A. Heinrichs, T. Huang, C. Berhin, P. Bogaerts, Y. Glupczynski] The purpose of this investigation was to compare several phenotypic methods, including combined disk tests (CDT) containing metallo-β-lactamase (MBL) inhibitors or cloxacillin, and the Carba NP test for the detection of carbapenemase-producing Pseudomonas aeruginosa (CPPA). A new CDT using imipenem (10μg) ± cloxacillin 4,000μg and the Carba NP test were evaluated to detect CPPA. In addition, four commercially available combined disks containing a carbapenem and ethylene-diamine-tetra-acetic acid (EDTA) or dipicolinic acid (DPA) as the inhibitor were tested in order to detect MBL-positive P. aeruginosa. All these phenotypic methods were evaluated on 188 imipenem non-susceptible P. aeruginosa (CPPA, n = 75) isolates divided into 26 well-characterized collection strains and 162 non-duplicate clinical isolates referred to the national reference laboratory in 2013. For the total of 188 isolates tested, CDT containing EDTA or DPA displayed high sensitivities (99%) and specificities (95%) for detecting MBL-producing isolates. CDT with cloxacillin showed a sensitivity and specificity of 97%/96% compared to 88%/99% for the Carba NP test in order to detect CPPA. For the 162 clinical isolates, CDT containing EDTA or DPA displayed a high negative predictive value (NPV) (99%) for detecting MBL-producing isolates. CDT with cloxacillin showed an NPV of 98%, compared to 95% for the Carba NP test in order to detect CPPA. In our setting, CDT associating imipenem ± EDTA or ± DPA performed best for the detection of MBL-producing P. aeruginosa. Imipenem/imipenem-cloxacillin test yielded good NPV to exclude the presence of MBL in imipenem non-susceptible isolates. Springer-Verlag Berlin Heidelberg, 2015 Heinrichs A. National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant Godinne, Université catholique de Louvain (UCL), Yvoir, Belgium aut Huang T. National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant Godinne, Université catholique de Louvain (UCL), Yvoir, Belgium aut Berhin C. National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant Godinne, Université catholique de Louvain (UCL), Yvoir, Belgium aut Bogaerts P. National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant Godinne, Université catholique de Louvain (UCL), Yvoir, Belgium aut Glupczynski Y. National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant Godinne, Université catholique de Louvain (UCL), Yvoir, Belgium aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/7(2015-07-01), 1467-1474 0934-9723 34:7<1467 2015 34 10096 https://doi.org/10.1007/s10096-015-2376-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2376-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Heinrichs A. National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant Godinne, Université catholique de Louvain (UCL), Yvoir, Belgium aut NATIONALLICENCE 700 1- Huang T. National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant Godinne, Université catholique de Louvain (UCL), Yvoir, Belgium aut NATIONALLICENCE 700 1- Berhin C. National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant Godinne, Université catholique de Louvain (UCL), Yvoir, Belgium aut NATIONALLICENCE 700 1- Bogaerts P. National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant Godinne, Université catholique de Louvain (UCL), Yvoir, Belgium aut NATIONALLICENCE 700 1- Glupczynski Y. National Reference Laboratory for Monitoring of Antimicrobial Resistance in Gram-Negative Bacteria, CHU Dinant Godinne, Université catholique de Louvain (UCL), Yvoir, Belgium aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/7(2015-07-01), 1467-1474 0934-9723 34:7<1467 2015 34 10096