caa a22 4500 606156267 CHVBK 20210128100607.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10096-015-2492-9 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2492-9 Rapid antimicrobial susceptibility testing of clinical isolates by digital time-lapse microscopy [Elektronische Daten] [M. Fredborg, F. Rosenvinge, E. Spillum, S. Kroghsbo, M. Wang, T. Sondergaard] Rapid antimicrobial susceptibility testing (AST) is essential for early and appropriate therapy. Methods with short detection time enabling same-day treatment optimisation are highly favourable. In this study, we evaluated the potential of a digital time-lapse microscope system, the oCelloScope system, to perform rapid AST. The oCelloScope system demonstrated a very high accuracy (96% overall agreement) when determining the resistance profiles of four reference strains, nine clinical isolates, including multi-drug-resistant isolates, and three positive blood cultures. AST of clinical isolates (168 antimicrobial agent-organism combinations) demonstrated 3.6% minor, no major and 1.2% very major errors of the oCelloScope system compared to conventional susceptibility testing, as well as a rapid and correct phenotypic detection of strains with methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum β-lactamase (ESBL) profiles. The net average time-to-result was 108min, with 95% of the results being available within 180min. In conclusion, this study strongly indicates that the oCelloScope system holds considerable potential as an accurate and sensitive AST method with short time-to-result, enabling same-day targeted antimicrobial therapy, facilitating antibiotic stewardship and better patient management. A full-scale validation of the oCelloScope system including more isolates is necessary to assess the impact of using it for AST. The Author(s), 2015 Fredborg M. Department of Animal Science, Faculty of Science and Technology, Aarhus University, Blichers Allé 20, 8830, Tjele, Denmark aut Rosenvinge F. Department of Clinical Microbiology, Vejle Hospital, Kabbeltoft 25, 7100, Vejle, Denmark aut Spillum E. Philips BioCell, Gydevang 42, 3450, Allerød, Denmark aut Kroghsbo S. Philips BioCell, Gydevang 42, 3450, Allerød, Denmark aut Wang M. Department of Clinical Microbiology, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200, Aarhus, Denmark aut Sondergaard T. Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, 9000, Aalborg, Denmark aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/12(2015-12-01), 2385-2394 0934-9723 34:12<2385 2015 34 10096 https://doi.org/10.1007/s10096-015-2492-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2492-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fredborg M. Department of Animal Science, Faculty of Science and Technology, Aarhus University, Blichers Allé 20, 8830, Tjele, Denmark aut NATIONALLICENCE 700 1- Rosenvinge F. Department of Clinical Microbiology, Vejle Hospital, Kabbeltoft 25, 7100, Vejle, Denmark aut NATIONALLICENCE 700 1- Spillum E. Philips BioCell, Gydevang 42, 3450, Allerød, Denmark aut NATIONALLICENCE 700 1- Kroghsbo S. Philips BioCell, Gydevang 42, 3450, Allerød, Denmark aut NATIONALLICENCE 700 1- Wang M. Department of Clinical Microbiology, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200, Aarhus, Denmark aut NATIONALLICENCE 700 1- Sondergaard T. Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, 9000, Aalborg, Denmark aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/12(2015-12-01), 2385-2394 0934-9723 34:12<2385 2015 34 10096