caa a22 4500 606156313 CHVBK 20210128100608.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10096-015-2490-y doi (NATIONALLICENCE)springer-10.1007/s10096-015-2490-y Vaginal microbiome and metabolome highlight specific signatures of bacterial vaginosis [Elektronische Daten] [B. Vitali, F. Cruciani, G. Picone, C. Parolin, G. Donders, L. Laghi] In this study, we sought to find novel bacterial and metabolic hallmarks for bacterial vaginosis (BV). We studied the vaginal microbiome and metabolome of vaginal fluids from BV-affected patients (n = 43) and healthy controls (n = 37) by means of an integrated approach based on quantitative polymerase chain reaction (qPCR) and proton nuclear magnetic resonance (1H-NMR). The correlations between the clinical condition and vaginal bacterial communities were investigated by principal component analysis (PCA). To define the metabolomics signatures of BV, 100 discriminant analysis by projection on latent structure (PLS-DA) models were calculated. Bacterial signatures distinguishing the health condition and BV were identified by qPCR. Lactobacillus crispatus strongly featured the healthy vagina, while increased concentrations of Prevotella, Atopobium and Mycoplasma hominis specifically marked the infection. 1H-NMR analysis has led to the identification and quantification of 17 previously unreported molecules. BV was associated with changes in the concentration of metabolites belonging to the families of amines, organic acids, short chain fatty acids, amino acids, nitrogenous bases and monosaccharides. In particular, maltose, kynurenine and NAD+ primarily characterised the healthy status, while nicotinate, malonate and acetate were the best metabolic hallmarks of BV. This study helps to better understand the role of the vaginal microbiota and metabolome in the development of BV infection. We propose a molecular approach for the diagnosis of BV based on quantitative detection in the vaginal fluids of Atopobium, Prevotella and M. hominis, and nicotinate, malonate and acetate by combining qPCR and 1H-NMR. Springer-Verlag Berlin Heidelberg, 2015 Vitali B. Department of Pharmacy and Biotechnology, University of Bologna, Via San Donato 19/2, 40127, Bologna, Italy aut Cruciani F. Department of Pharmacy and Biotechnology, University of Bologna, Via San Donato 19/2, 40127, Bologna, Italy aut Picone G. Department of Agro-Food Science and Technology, University of Bologna, P.zza Goidanich 60, 47522, Cesena, Italy aut Parolin C. Department of Pharmacy and Biotechnology, University of Bologna, Via San Donato 19/2, 40127, Bologna, Italy aut Donders G. Department of Obstetrics and Gynecology, General Hospital Heilig Hart, Kliniekstraat 45, 3300, Tienen, Belgium aut Laghi L. Department of Agro-Food Science and Technology, University of Bologna, P.zza Goidanich 60, 47522, Cesena, Italy aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/12(2015-12-01), 2367-2376 0934-9723 34:12<2367 2015 34 10096 https://doi.org/10.1007/s10096-015-2490-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2490-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vitali B. Department of Pharmacy and Biotechnology, University of Bologna, Via San Donato 19/2, 40127, Bologna, Italy aut NATIONALLICENCE 700 1- Cruciani F. Department of Pharmacy and Biotechnology, University of Bologna, Via San Donato 19/2, 40127, Bologna, Italy aut NATIONALLICENCE 700 1- Picone G. Department of Agro-Food Science and Technology, University of Bologna, P.zza Goidanich 60, 47522, Cesena, Italy aut NATIONALLICENCE 700 1- Parolin C. Department of Pharmacy and Biotechnology, University of Bologna, Via San Donato 19/2, 40127, Bologna, Italy aut NATIONALLICENCE 700 1- Donders G. Department of Obstetrics and Gynecology, General Hospital Heilig Hart, Kliniekstraat 45, 3300, Tienen, Belgium aut NATIONALLICENCE 700 1- Laghi L. Department of Agro-Food Science and Technology, University of Bologna, P.zza Goidanich 60, 47522, Cesena, Italy aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/12(2015-12-01), 2367-2376 0934-9723 34:12<2367 2015 34 10096