caa a22 4500 606156321 CHVBK 20210128100608.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s10096-015-2504-9 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2504-9 Microbial diversity on intravascular catheters from paediatric patients [Elektronische Daten] [L. Zhang, N. Marsh, D. Long, M. Wei, M. Morrison, C. Rickard] Microorganisms play important roles in intravascular catheter (IVC)-related infections, which are the most serious complications in children with IVCs, leading to increased hospitalisation, intensive care admissions, extensive antibiotic treatment and mortality. A greater understanding of bacterial communities is needed in order to improve the management of infections. We describe here the systematic culture-independent evaluation of IVC bacteriology in IVC biofilms. Twenty-four IVC samples (six peripherally inserted central catheters, eight central venous catheters and ten arterial catheters) were collected from 24 paediatric patients aged 0 to 14years old. Barcoded amplicon libraries produced from genes coding 16S rRNA and roll-plate culture methods were used to determine the microbial composition of these samples. From a total of 1,043,406 high-quality sequence reads, eight microbial phyla and 136 diverse microbial genera were detected, separated into 12,224 operational taxonomic units (OTUs). Three phyla (Actinobacteria, Firmicutes and Proteobacteria) predominate the microorganism on the IVC surfaces, with Firmicutes representing nearly half of the OTUs found. Among the Firmicutes, Staphylococcus (15.0% of 16S rRNA reads), Streptococcus (9.6%) and Bacillus (6.1%) were the most common. Community composition did not appear to be affected by patients' age, gender, antibiotic treatment or IVC type. Differences in IVC microbiota were more likely associated with events arising from catheter dwell time, rather than the type of IVC used. Springer-Verlag Berlin Heidelberg, 2015 Zhang L. AVATAR Group, Research Centre for Health Practice Innovation, Menzies Health Institute Queensland, Griffith University, Brisbane, Australia aut Marsh N. AVATAR Group, Research Centre for Health Practice Innovation, Menzies Health Institute Queensland, Griffith University, Brisbane, Australia aut Long D. AVATAR Group, Research Centre for Health Practice Innovation, Menzies Health Institute Queensland, Griffith University, Brisbane, Australia aut Wei M. School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast campus, Southport, Australia aut Morrison M. Translational Research Institute, The University of Queensland Diamantina Institute, Woolloongabba, Brisbane, Australia aut Rickard C. AVATAR Group, Research Centre for Health Practice Innovation, Menzies Health Institute Queensland, Griffith University, Brisbane, Australia aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/12(2015-12-01), 2463-2470 0934-9723 34:12<2463 2015 34 10096 https://doi.org/10.1007/s10096-015-2504-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2504-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang L. AVATAR Group, Research Centre for Health Practice Innovation, Menzies Health Institute Queensland, Griffith University, Brisbane, Australia aut NATIONALLICENCE 700 1- Marsh N. AVATAR Group, Research Centre for Health Practice Innovation, Menzies Health Institute Queensland, Griffith University, Brisbane, Australia aut NATIONALLICENCE 700 1- Long D. AVATAR Group, Research Centre for Health Practice Innovation, Menzies Health Institute Queensland, Griffith University, Brisbane, Australia aut NATIONALLICENCE 700 1- Wei M. School of Medical Science, Menzies Health Institute Queensland, Griffith University, Gold Coast campus, Southport, Australia aut NATIONALLICENCE 700 1- Morrison M. Translational Research Institute, The University of Queensland Diamantina Institute, Woolloongabba, Brisbane, Australia aut NATIONALLICENCE 700 1- Rickard C. AVATAR Group, Research Centre for Health Practice Innovation, Menzies Health Institute Queensland, Griffith University, Brisbane, Australia aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/12(2015-12-01), 2463-2470 0934-9723 34:12<2463 2015 34 10096