caa a22 4500 606156399 CHVBK 20210128100608.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10096-014-2250-4 doi (NATIONALLICENCE)springer-10.1007/s10096-014-2250-4 Evaluation of Verigene Gram-positive blood culture assay performance for bacteremic patients [Elektronische Daten] [M. Dodémont, R. De Mendonça, C. Nonhoff, S. Roisin, O. Denis] The Verigene Gram-Positive Blood Culture (BC-GP) Assay (Nanosphere Inc., Northbrook, IL) is a microarray-based test designed to rapidly identify directly from positive blood cultures multiple bacterial species and their antimicrobial resistance markers. Nonduplicate blood cultures from 118 patients admitted to Erasme Hospital were prospectively enrolled. All but six organisms were members of the panel (95.6%). For the identification of pathogens and detection of the mecA gene, the agreement with routine methods was 87.6% and 97.7%, respectively. The performance of the BC-GP assay was lower with polymicrobial than with monomicrobial blood cultures. Another concern of the BC-GP assay was the misidentification of Streptococcus mitis as S. pneumoniae (3/8). The BC-GP assay is a rapid and accurate tool for the simultaneous detection of multiple sepsis-causing bacteria and resistant genes from blood cultures, which could have an impact on patient management and healthcare cost. Springer-Verlag Berlin Heidelberg, 2014 Dodémont M. Laboratory of Bacteriology, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles (ULB), 808, route de Lennik, 1070, Brussels, Belgium aut De Mendonça R. Laboratory of Bacteriology, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles (ULB), 808, route de Lennik, 1070, Brussels, Belgium aut Nonhoff C. Laboratory of Bacteriology, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles (ULB), 808, route de Lennik, 1070, Brussels, Belgium aut Roisin S. Laboratory of Bacteriology, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles (ULB), 808, route de Lennik, 1070, Brussels, Belgium aut Denis O. Laboratory of Bacteriology, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles (ULB), 808, route de Lennik, 1070, Brussels, Belgium aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/3(2015-03-01), 473-477 0934-9723 34:3<473 2015 34 10096 https://doi.org/10.1007/s10096-014-2250-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2250-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dodémont M. Laboratory of Bacteriology, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles (ULB), 808, route de Lennik, 1070, Brussels, Belgium aut NATIONALLICENCE 700 1- De Mendonça R. Laboratory of Bacteriology, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles (ULB), 808, route de Lennik, 1070, Brussels, Belgium aut NATIONALLICENCE 700 1- Nonhoff C. Laboratory of Bacteriology, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles (ULB), 808, route de Lennik, 1070, Brussels, Belgium aut NATIONALLICENCE 700 1- Roisin S. Laboratory of Bacteriology, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles (ULB), 808, route de Lennik, 1070, Brussels, Belgium aut NATIONALLICENCE 700 1- Denis O. Laboratory of Bacteriology, Department of Microbiology, Hôpital Erasme, Université Libre de Bruxelles (ULB), 808, route de Lennik, 1070, Brussels, Belgium aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/3(2015-03-01), 473-477 0934-9723 34:3<473 2015 34 10096