caa a22 4500 606156402 CHVBK 20210128100608.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10096-014-2255-z doi (NATIONALLICENCE)springer-10.1007/s10096-014-2255-z Evaluation of a new chromogenic medium, chromID OXA-48, for recovery of carbapenemase-producing Enterobacteriaceae from patients at a university hospital in Turkey [Elektronische Daten] [P. Zarakolu, K. Day, H. Sidjabat, W. Kamolvit, C. Lanyon, S. Cummings, D. Paterson, M. Akova, J. Perry] The purpose of this study was to evaluate a new chromogenic medium, chromID OXA-48, for the isolation of carbapenemase-producing Enterobacteriaceae (CPE) directly from rectal swabs. chromID CARBA and chromID OXA-48 are two chromogenic media that have been commercialized for the isolation of CPE directly from clinical samples. Both media were evaluated alongside a broth enrichment method recommended by the CDC for isolation of CPE, with rectal swabs from 302 unique hospitalized patients at the Hacettepe University Hospital, Ankara, Turkey. A total of 33 patients (11%) were found to be colonized with CPE using a combination of all methods, and all CPE produced OXA-48 carbapenemase. Klebsiella pneumoniae was by far the most dominant species of CPE and was isolated from 31 patients. Culture on chromID OXA-48 offered the highest sensitivity (75.8%) for detection of CPE compared with the other two methods (sensitivity for both other methods was 57.6%) and also offered the highest specificity (99.3%). However, a combination of methods (either chromID OXA-48 plus CDC method or chromID OXA-48 plus chromID CARBA) was necessary to achieve an acceptable sensitivity (90.9%). For isolation of CPE, in a setting where OXA-48 carbapenemase is the dominant type of carbapenemase, chromID OXA-48 is a highly useful medium but using a combination of methods is optimal for adequate detection. The combined use of two chromogenic media offered acceptable sensitivity (90.9%) and the highest specificity (98.5%) and also allowed for isolation of CPE within 18-20h. Springer-Verlag Berlin Heidelberg, 2014 Zarakolu P. Department of Infectious Diseases and Clinical Microbiology, Hacettepe University School of Medicine, 06100, Ankara, Turkey aut Day K. Faculty of Health and Life Sciences, Northumbria University, NE1 8ST, Newcastle upon Tyne, UK aut Sidjabat H. University of Queensland Centre for Clinical Research, Brisbane, Queensland, Australia aut Kamolvit W. University of Queensland Centre for Clinical Research, Brisbane, Queensland, Australia aut Lanyon C. Faculty of Health and Life Sciences, Northumbria University, NE1 8ST, Newcastle upon Tyne, UK aut Cummings S. Faculty of Health and Life Sciences, Northumbria University, NE1 8ST, Newcastle upon Tyne, UK aut Paterson D. University of Queensland Centre for Clinical Research, Brisbane, Queensland, Australia aut Akova M. Department of Infectious Diseases and Clinical Microbiology, Hacettepe University School of Medicine, 06100, Ankara, Turkey aut Perry J. Faculty of Health and Life Sciences, Northumbria University, NE1 8ST, Newcastle upon Tyne, UK aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/3(2015-03-01), 519-525 0934-9723 34:3<519 2015 34 10096 https://doi.org/10.1007/s10096-014-2255-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2255-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zarakolu P. Department of Infectious Diseases and Clinical Microbiology, Hacettepe University School of Medicine, 06100, Ankara, Turkey aut NATIONALLICENCE 700 1- Day K. Faculty of Health and Life Sciences, Northumbria University, NE1 8ST, Newcastle upon Tyne, UK aut NATIONALLICENCE 700 1- Sidjabat H. University of Queensland Centre for Clinical Research, Brisbane, Queensland, Australia aut NATIONALLICENCE 700 1- Kamolvit W. University of Queensland Centre for Clinical Research, Brisbane, Queensland, Australia aut NATIONALLICENCE 700 1- Lanyon C. Faculty of Health and Life Sciences, Northumbria University, NE1 8ST, Newcastle upon Tyne, UK aut NATIONALLICENCE 700 1- Cummings S. Faculty of Health and Life Sciences, Northumbria University, NE1 8ST, Newcastle upon Tyne, UK aut NATIONALLICENCE 700 1- Paterson D. University of Queensland Centre for Clinical Research, Brisbane, Queensland, Australia aut NATIONALLICENCE 700 1- Akova M. Department of Infectious Diseases and Clinical Microbiology, Hacettepe University School of Medicine, 06100, Ankara, Turkey aut NATIONALLICENCE 700 1- Perry J. Faculty of Health and Life Sciences, Northumbria University, NE1 8ST, Newcastle upon Tyne, UK aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/3(2015-03-01), 519-525 0934-9723 34:3<519 2015 34 10096