caa a22 4500 606156453 CHVBK 20210128100608.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10096-014-2244-2 doi (NATIONALLICENCE)springer-10.1007/s10096-014-2244-2 Molecular characterization of the extended-spectrum beta-lactamase (ESBL)-producing Shigella spp. in Shanghai [Elektronische Daten] [J. Li, B. Li, Y. Ni, J. Sun] Shigellosis is a public health concern in China. We tested 216 Shigella isolates collected in Shanghai in 2007 for the production of extended-spectrum beta-lactamases (ESBLs). ESBL-producing isolates were characterized using polymerase chain reaction (PCR)-based genotyping, conjugation, pulsed-field gel electrophoresis (PFGE), and DNA sequence analysis of regions adjacent to bla genes. Plasmids containing genes encoding ESBLs were analyzed using plasmid replicon typing. ESBLs were produced by 18.1% (39/216) of Shigella isolates, and all 39 ESBL-producing strains harbored bla CTX-M genes. CTX-M-14 was the most frequent variant (69.2%, 27/39), followed by CTX-M-15 (15.4%, 6/39). All bla CTX-M genes were transferable by conjugation, and the insertion sequence ISEcp1 was detected upstream of all bla CTX-M genes. The CTX-M-producing Shigella isolates showed high clonal diversity. IncI1, IncFII, IncN, and IncB/O replicons were respectively detected in 23 (58.9%), 9 (23.1%), 1 (2.6%), and 1 (2.6%) of the 39 transconjugants carrying bla CTX-M. The bla CTX-M-14 genes were most frequently carried by IncI1 (n = 13, 48.1%) or IncFII (n = 9, 33.3%) plasmids, and the bla CTX-M-15 genes were closely associated with IncI1 (n = 5, 83.3%). Our findings demonstrate the high prevalence of ESBL-producing Shigella in Shanghai, the importance of plasmids and ISEcp1 as carriers of bla CTX-M genes, and the close association between certain bla CTX-M genes with a specific plasmid. Springer-Verlag Berlin Heidelberg, 2014 Li J. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin 2nd Rd, 200025, Shanghai, China aut Li B. Department of Clinical Laboratory, Union Hospital, Fujian Medical University, 350001, Fuzhou, Fujian, China aut Ni Y. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin 2nd Rd, 200025, Shanghai, China aut Sun J. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin 2nd Rd, 200025, Shanghai, China aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/3(2015-03-01), 447-451 0934-9723 34:3<447 2015 34 10096 https://doi.org/10.1007/s10096-014-2244-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2244-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li J. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin 2nd Rd, 200025, Shanghai, China aut NATIONALLICENCE 700 1- Li B. Department of Clinical Laboratory, Union Hospital, Fujian Medical University, 350001, Fuzhou, Fujian, China aut NATIONALLICENCE 700 1- Ni Y. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin 2nd Rd, 200025, Shanghai, China aut NATIONALLICENCE 700 1- Sun J. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin 2nd Rd, 200025, Shanghai, China aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/3(2015-03-01), 447-451 0934-9723 34:3<447 2015 34 10096