caa a22 4500 60615650X CHVBK 20210128100609.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10096-014-2266-9 doi (NATIONALLICENCE)springer-10.1007/s10096-014-2266-9 Mixed infections with distinct cytomegalovirus glycoprotein B genotypes in Polish pregnant women, fetuses, and newborns [Elektronische Daten] [M. Rycel, W. Wujcicka, B. Zawilińska, E. Paradowska, P. Suski, Z. Gaj, J. Wilczyński, Z. Leśnikowski, D. Nowakowska] The purpose of this investigation was to describe a distribution of cytomegalovirus (CMV) single and multiple genotypes among infected pregnant women, their fetuses, and newborns coming from Central Poland, as well as congenital cytomegaly outcome. The study involved 278 CMV-seropositive pregnant women, of whom 192 were tested for viral DNAemia. Human cytomegalovirus (HCMV) genotyping was performed for 18 of 34 pregnant women carrying the viral DNA and for 12 of their 15 offspring with confirmed HCMV infections. Anti-HCMV antibodies levels were assessed by chemiluminescence immunoassay (CLIA) and enzyme-linked fluorescence assay (ELFA) tests. Viral DNA loads and genotypes were determined by real-time polymerase chain reaction (PCR) assays for the UL55 gene. In the pregnant women, we identified HCMV gB1, gB2, gB3, and gB4 genotypes. Single gB2, gB3, or gB4 genotypes were observed in 14 (77.8 %) women, while multiple gB1-gB2 or gB2-gB3 genotypes were observed in four (22.2 %). Maternal HCMV genotypes determined the genotypes identified in their fetuses and newborns (p ≤ 0.050). Half of them were infected with single HCMV gB1, gB2, or gB3 genotypes and the other half with multiple gB1-gB2 or gB2-gB3 genotypes. Single and multiple genotypes were observed in both asymptomatic and symptomatic congenital cytomegaly, although no gB3 genotype was identified among asymptomatic cases. In Central Poland, infections with single and multiple HCMV strains occur in pregnant women, as well as in their fetuses and neonates, with both asymptomatic and symptomatic infections. HCMV infections identified in mothers seem to be associated with the viral genotypes in their children. The Author(s), 2014 Rycel M. Department of Gynecology and Gynecologic Oncology, Polish Mother's Memorial Hospital Research Institute, 281/289 Rzgowska Street, 93-338, Lodz, Poland aut Wujcicka W. Department of Fetal-Maternal Medicine and Gynecology, Polish Mother's Memorial Hospital Research Institute, 281/289 Rzgowska Street, 93-338, Lodz, Poland aut Zawilińska B. Department of Virology, Jagiellonian University Medical College, Cracow, Poland aut Paradowska E. Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland aut Suski P. Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland aut Gaj Z. Department of Fetal-Maternal Medicine and Gynecology, Polish Mother's Memorial Hospital Research Institute, 281/289 Rzgowska Street, 93-338, Lodz, Poland aut Wilczyński J. Department of Fetal-Maternal Medicine and Gynecology, Polish Mother's Memorial Hospital Research Institute, 281/289 Rzgowska Street, 93-338, Lodz, Poland aut Leśnikowski Z. Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland aut Nowakowska D. Department of Fetal-Maternal Medicine and Gynecology, Polish Mother's Memorial Hospital Research Institute, 281/289 Rzgowska Street, 93-338, Lodz, Poland aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/3(2015-03-01), 585-591 0934-9723 34:3<585 2015 34 10096 https://doi.org/10.1007/s10096-014-2266-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2266-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rycel M. Department of Gynecology and Gynecologic Oncology, Polish Mother's Memorial Hospital Research Institute, 281/289 Rzgowska Street, 93-338, Lodz, Poland aut NATIONALLICENCE 700 1- Wujcicka W. Department of Fetal-Maternal Medicine and Gynecology, Polish Mother's Memorial Hospital Research Institute, 281/289 Rzgowska Street, 93-338, Lodz, Poland aut NATIONALLICENCE 700 1- Zawilińska B. Department of Virology, Jagiellonian University Medical College, Cracow, Poland aut NATIONALLICENCE 700 1- Paradowska E. Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland aut NATIONALLICENCE 700 1- Suski P. Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland aut NATIONALLICENCE 700 1- Gaj Z. Department of Fetal-Maternal Medicine and Gynecology, Polish Mother's Memorial Hospital Research Institute, 281/289 Rzgowska Street, 93-338, Lodz, Poland aut NATIONALLICENCE 700 1- Wilczyński J. Department of Fetal-Maternal Medicine and Gynecology, Polish Mother's Memorial Hospital Research Institute, 281/289 Rzgowska Street, 93-338, Lodz, Poland aut NATIONALLICENCE 700 1- Leśnikowski Z. Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland aut NATIONALLICENCE 700 1- Nowakowska D. Department of Fetal-Maternal Medicine and Gynecology, Polish Mother's Memorial Hospital Research Institute, 281/289 Rzgowska Street, 93-338, Lodz, Poland aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/3(2015-03-01), 585-591 0934-9723 34:3<585 2015 34 10096