caa a22 4500 606156615 CHVBK 20210128100609.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10096-014-2272-y doi (NATIONALLICENCE)springer-10.1007/s10096-014-2272-y Tigecycline resistance in Acinetobacter baumannii mediated by frameshift mutation in plsC , encoding 1-acyl-sn-glycerol-3-phosphate acyltransferase [Elektronische Daten] [X. Li, L. Liu, J. Ji, Q. Chen, X. Hua, Y. Jiang, Y. Feng, Y. Yu] Acinetobacter baumannii is an important pathogen of healthcare-associated infections and shows multidrug resistance. With the increasing application of tigecycline, isolates resistant to this antibiotic are of growing concern clinically. However, the definitive mechanism of tigecycline resistance remains unclear. To explore the mechanism of tigecycline resistance in A. baumannii, a tigecycline-resistant strain was obtained by increasing the concentration of the antimicrobial in liquid culture. Three mutations were identified by the whole genome comparison, including one synonymous substitution in a hypothetical protein and a frameshift mutation in plsC and omp38. The plsC gene was confirmed to cause decreased susceptibility to tigecycline by a complementation experiment and cellular membrane change was detected by flow cytometry. By measuring the relative growth rate, the fitness cost of plsC was estimated to be approximately 8%. In conclusion, plsC was found to play an important role in tigecycline resistance in A. baumannii. The minor fitness cost of plsC indicates a high risk of the emergence and development of tigecycline resistance in A. baumannii. Springer-Verlag Berlin Heidelberg, 2014 Li X. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut Liu L. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut Ji J. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut Chen Q. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut Hua X. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut Jiang Y. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut Feng Y. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut Yu Y. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/3(2015-03-01), 625-631 0934-9723 34:3<625 2015 34 10096 https://doi.org/10.1007/s10096-014-2272-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2272-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li X. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Liu L. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Ji J. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Chen Q. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Hua X. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Jiang Y. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Feng Y. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut NATIONALLICENCE 700 1- Yu Y. Department of Infectious Diseases, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, 310016, Hangzhou, Zhejiang, China aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/3(2015-03-01), 625-631 0934-9723 34:3<625 2015 34 10096