caa a22 4500 60615664X CHVBK 20210128100609.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10096-015-2458-y doi (NATIONALLICENCE)springer-10.1007/s10096-015-2458-y Direct identification of major Gram-negative pathogens in respiratory specimens by respiFISH® HAP Gram (−) Panel, a beacon-based FISH methodology [Elektronische Daten] [R. Koncan, M. Parisato, C. Sakarikou, G. Stringari, C. Fontana, V. Favuzzi, M. Ligozzi, G. Lo Cascio] Rapid detection of microorganisms in respiratory specimens is of paramount importance to drive the proper antibiotic regimen to prevent complications and transmission of infections. In the present study, the respiFISH® HAP Gram (−) Panel (miacom diagnostics GmbH, Duesseldorf, Germany) for the etiological diagnosis of hospital-acquired pneumonia was compared with the traditional culture method for the detection of major Gram-negative pathogens in respiratory specimens. respiFISH® combined the classical fluorescence in situ hybridization (FISH) technology with fluorescence-labeled DNA molecular beacons as probes. From September 2011 to January 2012, 165 samples were analyzed: the sensitivity and specificity were 94.39 and 87.93%, respectively. Only six pathogens (3.6%) were not identified with respiFISH®, while seven specimens (3%) provided false-positive results. This beacon-based identification shortens the time to result by at least one work day, providing species-level identification within half an hour. Considering the high sensitivity and specificity and the significant time saving, the introduction of bbFISH® assays could effectively complement traditional systems in microbiology laboratories. Springer-Verlag Berlin Heidelberg, 2015 Koncan R. Department of Pathology and Diagnostics, University of Verona, Strada Le Grazie 8, 37134, Verona, Italy aut Parisato M. O.U. of Microbiology and Virology, Department of Pathology and Diagnostics, Azienda Ospedaliera Universitaria Integrata di Verona, P.le A. Scuro 10, 37134, Verona, Italy aut Sakarikou C. Clinical Microbiology Laboratories, Foundation Polyclinic Tor Vergata, V.le Oxford 81, 00133, Roma, Italy aut Stringari G. Department of Anesthesiological Sciences and Specialistic Surgeries, University of Verona, P.le A. Scuro 10, 37134, Verona, Italy aut Fontana C. Clinical Microbiology Laboratories, Foundation Polyclinic Tor Vergata, V.le Oxford 81, 00133, Roma, Italy aut Favuzzi V. Department of Pathology and Diagnostics, University of Verona, Strada Le Grazie 8, 37134, Verona, Italy aut Ligozzi M. Department of Pathology and Diagnostics, University of Verona, Strada Le Grazie 8, 37134, Verona, Italy aut Lo Cascio G. O.U. of Microbiology and Virology, Department of Pathology and Diagnostics, Azienda Ospedaliera Universitaria Integrata di Verona, P.le A. Scuro 10, 37134, Verona, Italy aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/10(2015-10-01), 2097-2102 0934-9723 34:10<2097 2015 34 10096 https://doi.org/10.1007/s10096-015-2458-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2458-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koncan R. Department of Pathology and Diagnostics, University of Verona, Strada Le Grazie 8, 37134, Verona, Italy aut NATIONALLICENCE 700 1- Parisato M. O.U. of Microbiology and Virology, Department of Pathology and Diagnostics, Azienda Ospedaliera Universitaria Integrata di Verona, P.le A. Scuro 10, 37134, Verona, Italy aut NATIONALLICENCE 700 1- Sakarikou C. Clinical Microbiology Laboratories, Foundation Polyclinic Tor Vergata, V.le Oxford 81, 00133, Roma, Italy aut NATIONALLICENCE 700 1- Stringari G. Department of Anesthesiological Sciences and Specialistic Surgeries, University of Verona, P.le A. Scuro 10, 37134, Verona, Italy aut NATIONALLICENCE 700 1- Fontana C. Clinical Microbiology Laboratories, Foundation Polyclinic Tor Vergata, V.le Oxford 81, 00133, Roma, Italy aut NATIONALLICENCE 700 1- Favuzzi V. Department of Pathology and Diagnostics, University of Verona, Strada Le Grazie 8, 37134, Verona, Italy aut NATIONALLICENCE 700 1- Ligozzi M. Department of Pathology and Diagnostics, University of Verona, Strada Le Grazie 8, 37134, Verona, Italy aut NATIONALLICENCE 700 1- Lo Cascio G. O.U. of Microbiology and Virology, Department of Pathology and Diagnostics, Azienda Ospedaliera Universitaria Integrata di Verona, P.le A. Scuro 10, 37134, Verona, Italy aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/10(2015-10-01), 2097-2102 0934-9723 34:10<2097 2015 34 10096