caa a22 4500 606156666 CHVBK 20210128100609.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10096-015-2443-5 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2443-5 Effects of near-infrared laser radiation on the survival and inflammatory potential of Candida spp. involved in the pathogenesis of chemotherapy-induced oral mucositis [Elektronische Daten] [A. Clemente, L. Rizzetto, G. Castronovo, E. Perissi, M. Tanturli, F. Cozzolino, D. Cavalieri, F. Fusi, F. Cialdai, L. Vignali, M. Torcia, M. Monici] Candida spp. usually colonize ulcerative lesions of atrophic mucosa in patients with chemotherapy-induced oral mucositis inducing severe inflammation. The spread of antifungal-resistant strains strongly encouraged the search of complementary or alternative therapeutic strategies to cure inflamed mucosa. In this paper, we studied the effects of a near-infrared (NIR) laser system with dual-wavelength emission (808nm + 904nm) on the survival and inflammatory potential of C. albicans, C. glabrata, and C. parapsilosis. Laser treatment was performed with a Multiwave Locked System laser. Survival and apoptosis of fungal strains were evaluated by colony-forming units (CFU) counting and annexin V staining. Cytokine production was evaluated by ImmunoPlex array. Laser treatment significantly affected the survival of Candida spp. by inducing apoptosis and induced a lower production of inflammatory cytokines by dendritic cells compared to untreated fungi. No differences in the survival and inflammatory potential were recorded in treated or untreated Saccharomyces cerevisiae cells, used as the control non-pathogenic microorganism. Laser treatment altered the survival and inflammatory potential of pathogenic Candida spp. These data provide experimental support to the use of NIR laser radiation as a co-adjuvant of antifungal therapy in patients with oral mucositis (OM) complicated by Candida infections. Springer-Verlag Berlin Heidelberg, 2015 Clemente A. Department of Clinical and Experimental Medicine, University of Firenze, Firenze, FI, Italy aut Rizzetto L. Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, TN, Italy aut Castronovo G. Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut Perissi E. Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut Tanturli M. Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut Cozzolino F. Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut Cavalieri D. Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, TN, Italy aut Fusi F. Medical Physics Unit, Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut Cialdai F. ASAcampus Joint Laboratory, ASA Research Division, Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut Vignali L. ASAcampus Joint Laboratory, ASA Research Division, Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut Torcia M. Department of Clinical and Experimental Medicine, University of Firenze, Firenze, FI, Italy aut Monici M. ASAcampus Joint Laboratory, ASA Research Division, Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/10(2015-10-01), 1999-2007 0934-9723 34:10<1999 2015 34 10096 https://doi.org/10.1007/s10096-015-2443-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2443-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Clemente A. Department of Clinical and Experimental Medicine, University of Firenze, Firenze, FI, Italy aut NATIONALLICENCE 700 1- Rizzetto L. Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, TN, Italy aut NATIONALLICENCE 700 1- Castronovo G. Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut NATIONALLICENCE 700 1- Perissi E. Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut NATIONALLICENCE 700 1- Tanturli M. Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut NATIONALLICENCE 700 1- Cozzolino F. Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut NATIONALLICENCE 700 1- Cavalieri D. Research and Innovation Centre, Fondazione Edmund Mach, San Michele all'Adige, TN, Italy aut NATIONALLICENCE 700 1- Fusi F. Medical Physics Unit, Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut NATIONALLICENCE 700 1- Cialdai F. ASAcampus Joint Laboratory, ASA Research Division, Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut NATIONALLICENCE 700 1- Vignali L. ASAcampus Joint Laboratory, ASA Research Division, Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut NATIONALLICENCE 700 1- Torcia M. Department of Clinical and Experimental Medicine, University of Firenze, Firenze, FI, Italy aut NATIONALLICENCE 700 1- Monici M. ASAcampus Joint Laboratory, ASA Research Division, Department of Experimental and Clinical Biomedical Sciences, University of Firenze, Firenze, FI, Italy aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/10(2015-10-01), 1999-2007 0934-9723 34:10<1999 2015 34 10096