caa a22 4500 60615681X CHVBK 20210128100610.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10096-015-2440-8 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2440-8 The speciation and genotyping of Cronobacter isolates from hospitalised patients [Elektronische Daten] [A. Alsonosi, S. Hariri, M. Kajsík, M. Oriešková, V. Hanulík, M. Röderová, J. Petrželová, H. Kollárová, H. Drahovská, S. Forsythe, O. Holý] The World Health Organization (WHO) has recognised all Cronobacter species as human pathogens. Among premature neonates and immunocompromised infants, these infections can be life-threatening, with clinical presentations of septicaemia, meningitis and necrotising enterocolitis. The neurological sequelae can be permanent and the mortality rate as high as 40-80%. Despite the highlighted issues of neonatal infections, the majority of Cronobacter infections are in the elderly population suffering from serious underlying disease or malignancy and include wound and urinary tract infections, osteomyelitis, bacteraemia and septicaemia. However, no age profiling studies have speciated or genotyped the Cronobacter isolates. A clinical collection of 51 Cronobacter strains from two hospitals were speciated and genotyped using 7-loci multilocus sequence typing (MLST), rpoB gene sequence analysis, O-antigen typing and pulsed-field gel electrophoresis (PFGE). The isolates were predominated by C. sakazakii sequence type 4 (63%, 32/51) and C. malonaticus sequence type 7 (33%, 17/51). These had been isolated from throat and sputum samples of all age groups, as well as recal and faecal swabs. There was no apparent relatedness between the age of the patient and the Cronobacter species isolated. Despite the high clonality of Cronobacter, PFGE profiles differentiated strains across the sequence types into 15 pulsotypes. There was almost complete agreement between O-antigen typing and rpoB gene sequence analysis and MLST profiling. This study shows the value of applying MLST to bacterial population studies with strains from two patient cohorts, combined with PFGE for further discrimination of strains. The Author(s), 2015 Alsonosi A. Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Clifton Lane, NG11 8NS, Nottingham, UK aut Hariri S. Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Clifton Lane, NG11 8NS, Nottingham, UK aut Kajsík M. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia aut Oriešková M. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia aut Hanulík V. Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic aut Röderová M. Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic aut Petrželová J. Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic aut Kollárová H. Department of Preventive Medicine, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic aut Drahovská H. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia aut Forsythe S. Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Clifton Lane, NG11 8NS, Nottingham, UK aut Holý O. Department of Preventive Medicine, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/10(2015-10-01), 1979-1988 0934-9723 34:10<1979 2015 34 10096 https://doi.org/10.1007/s10096-015-2440-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2440-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alsonosi A. Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Clifton Lane, NG11 8NS, Nottingham, UK aut NATIONALLICENCE 700 1- Hariri S. Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Clifton Lane, NG11 8NS, Nottingham, UK aut NATIONALLICENCE 700 1- Kajsík M. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Oriešková M. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Hanulík V. Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Röderová M. Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Petrželová J. Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Kollárová H. Department of Preventive Medicine, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Drahovská H. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia aut NATIONALLICENCE 700 1- Forsythe S. Pathogen Research Group, School of Science and Technology, Nottingham Trent University, Clifton Lane, NG11 8NS, Nottingham, UK aut NATIONALLICENCE 700 1- Holý O. Department of Preventive Medicine, Faculty of Medicine and Dentistry, Palacký University, Olomouc, Olomouc, Czech Republic aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/10(2015-10-01), 1979-1988 0934-9723 34:10<1979 2015 34 10096