caa a22 4500 606156828 CHVBK 20210128100610.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10096-015-2460-4 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2460-4 Analytic laboratory performance of a point of care urine culture kit for diagnosis and antibiotic susceptibility testing [Elektronische Daten] [E. Bongard, N. Frimodt-Møller, M. Gal, M. Wootton, R. Howe, N. Francis, H. Goossens, C. Butler] Currently available point-of-care (POC) diagnostic tests for managing urinary tract infections (UTIs) in general practice are limited by poor performance characteristics, and laboratory culture generally provides results only after a few days. This laboratory evaluation compared the analytic performance of the POC UK Flexicult™ (Statens Serum Institut) (SSI) urinary kit for quantification, identification and antibiotic susceptibility testing and routine UK National Health Service (NHS) urine processing to an advanced urine culture method. Two hundred urine samples routinely submitted to the Public Health Wales Microbiology Laboratory were divided and: (1) analysed by routine NHS microbiological tests as per local laboratory standard operating procedures, (2) inoculated onto the UK Flexicult™ SSI urinary kit and (3) spiral plated onto Colorex Orientation UTI medium (E&O Laboratories Ltd). The results were evaluated between the NHS and Flexicult™ methods, and discordant results were compared to the spiral plating method. The UK Flexicult™ SSI urinary kit was compared to routine NHS culture for identification of a pure or predominant uropathogen at ≥105cfu/mL, with a positive discordancy rate of 13.5% and a negative discordancy rate of 3%. The sensitivity and specificity were 86.7% [95% confidence interval (CI) 73.8-93.7] and 82.6% (95% CI 75.8-87.7), respectively. The UK Flexicult™ SSI urinary kit was comparable to routine NHS urine processing in identifying microbiologically positive UTIs in this laboratory evaluation. However, the number of false-positive samples could lead to over-prescribing of antibiotics in clinical practice. The Flexicult™ SSI kit could be useful as a POC test for UTIs in primary care but further pragmatic evaluations are necessary. Springer-Verlag Berlin Heidelberg, 2015 Bongard E. Cardiff University, Cardiff, Wales aut Frimodt-Møller N. Statens Serum Institut, Copenhagen, Denmark aut Gal M. Cardiff University, Cardiff, Wales aut Wootton M. Public Health Wales, Cardiff, Wales aut Howe R. Public Health Wales, Cardiff, Wales aut Francis N. Cardiff University, Cardiff, Wales aut Goossens H. University of Antwerp, Antwerpen, Belgium aut Butler C. Cardiff University, Cardiff, Wales aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/10(2015-10-01), 2111-2119 0934-9723 34:10<2111 2015 34 10096 https://doi.org/10.1007/s10096-015-2460-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2460-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bongard E. Cardiff University, Cardiff, Wales aut NATIONALLICENCE 700 1- Frimodt-Møller N. Statens Serum Institut, Copenhagen, Denmark aut NATIONALLICENCE 700 1- Gal M. Cardiff University, Cardiff, Wales aut NATIONALLICENCE 700 1- Wootton M. Public Health Wales, Cardiff, Wales aut NATIONALLICENCE 700 1- Howe R. Public Health Wales, Cardiff, Wales aut NATIONALLICENCE 700 1- Francis N. Cardiff University, Cardiff, Wales aut NATIONALLICENCE 700 1- Goossens H. University of Antwerp, Antwerpen, Belgium aut NATIONALLICENCE 700 1- Butler C. Cardiff University, Cardiff, Wales aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/10(2015-10-01), 2111-2119 0934-9723 34:10<2111 2015 34 10096