caa a22 4500 606156860 CHVBK 20210128100610.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10096-015-2438-2 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2438-2 Rapid susceptibility testing for slowly growing nontuberculous mycobacteria using a colorimetric microbial viability assay based on the reduction of water-soluble tetrazolium WST-1 [Elektronische Daten] [T. Tsukatani, H. Suenaga, M. Shiga, T. Ikegami, M. Ishiyama, T. Ezoe, K. Matsumoto] Rapid susceptibility testing for slowly growing nontuberculous mycobacteria (NTM) using a colorimetric microbial viability assay based on the reduction of the water-soluble tetrazolium salt {2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt (WST-1)} using 2,3,5,6-tetramethyl-1,4-benzoquinone as an electron mediator was developed. Using the Clinical and Laboratory Standards Institute (CLSI) method, a long-term incubation time (7-14days) was required to determine the minimum inhibitory concentrations (MICs) of the slowly growing NTM. The MICs for a variety of different antibiotics against the slowly growing NTM were determined by the WST-1 colorimetric method and compared with those obtained using the broth microdilution methods approved by the CLSI. Good agreement was found between the MICs determined after 3-4days using the WST-1 colorimetric method and those obtained after 10-14days using the broth microdilution method. The results suggest that the WST-1 colorimetric assay is a useful method for the rapid determination of the MICs for the slowly growing NTM. Springer-Verlag Berlin Heidelberg, 2015 Tsukatani T. Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa-machi, 839-0861, Kurume, Japan aut Suenaga H. Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa-machi, 839-0861, Kurume, Japan aut Shiga M. Dojindo Laboratories, 861-2202, Kumamoto, Japan aut Ikegami T. Dojindo Laboratories, 861-2202, Kumamoto, Japan aut Ishiyama M. Dojindo Laboratories, 861-2202, Kumamoto, Japan aut Ezoe T. Dojindo Laboratories, 861-2202, Kumamoto, Japan aut Matsumoto K. Laboratory of Food Bioscience, Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, 860-0082, Kumamoto, Japan aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/10(2015-10-01), 1965-1973 0934-9723 34:10<1965 2015 34 10096 https://doi.org/10.1007/s10096-015-2438-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2438-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tsukatani T. Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa-machi, 839-0861, Kurume, Japan aut NATIONALLICENCE 700 1- Suenaga H. Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa-machi, 839-0861, Kurume, Japan aut NATIONALLICENCE 700 1- Shiga M. Dojindo Laboratories, 861-2202, Kumamoto, Japan aut NATIONALLICENCE 700 1- Ikegami T. Dojindo Laboratories, 861-2202, Kumamoto, Japan aut NATIONALLICENCE 700 1- Ishiyama M. Dojindo Laboratories, 861-2202, Kumamoto, Japan aut NATIONALLICENCE 700 1- Ezoe T. Dojindo Laboratories, 861-2202, Kumamoto, Japan aut NATIONALLICENCE 700 1- Matsumoto K. Laboratory of Food Bioscience, Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, 860-0082, Kumamoto, Japan aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/10(2015-10-01), 1965-1973 0934-9723 34:10<1965 2015 34 10096