caa a22 4500 606156895 CHVBK 20210128100610.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10096-015-2451-5 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2451-5 In vitro antimicrobial susceptibility of Helcococcus kunzii and molecular analysis of macrolide and tetracycline resistance [Elektronische Daten] [A. Vergne, F. Guérin, R. Lienhard, A. Le Coustumier, C. Daurel, C. Isnard, N. Marty, C. Poyart, V. Cattoir] Thanks to the recent advent of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) technology, Helcococcus kunzii is now easily identifiable and considered as an opportunistic pathogen. However, data about antimicrobial susceptibilities remain very limited. The aim of the study was, then, to assess its in vitro susceptibility to 18 antimicrobial agents and to investigate the genetic basis of macrolide and tetracycline resistance. Thirty-nine human clinical isolates of H. kunzii collected from 2008 to 2013 were studied, as well as the type strain ATCC 51366T. Minimum inhibitory concentrations (MICs) of penicillin G, amoxicillin, cefotaxime, imipenem, gentamicin, erythromycin, clindamycin, quinupristin-dalfopristin, ciprofloxacin, levofloxacin, tetracycline, tigecycline, vancomycin, teicoplanin, linezolid, daptomycin, cotrimoxazole and rifampin were determined by the microdilution method. Screening for macrolide [erm(A) including erm(TR), erm(B), erm(C), erm(F), erm(T), erm(X), msr(A) and mef(A)] and tetracycline [tet(L), tet(M) and tet(O)] resistance genes was performed, as well as the detection of mutations in 23S rRNA. Except for one strain resistant to cefotaxime, all strains were categorised as susceptible to β-lactams, glycopeptides, linezolid, daptomycin and tigecycline. Whereas ciprofloxacin and gentamicin exhibited limited activity, 95% of strains were categorised as susceptible to levofloxacin. Concerning erythromycin, a bimodal distribution was observed, with 29 ‘wild-type' strains (MICs from 0.25 to 2mg/L) and 11 ‘resistant' strains (MICs ≥256mg/L), including ten harbouring erm(TR). Two isolates exhibited acquired tetracycline resistance (MICs of 16mg/L) by the production of tet(M). This large study on the in vitro antimicrobial susceptibility of H. kunzii suggests that β-lactams (especially penicillins) should be preferred for the treatment. Springer-Verlag Berlin Heidelberg, 2015 Vergne A. Laboratoire de Biologie, CH de Cahors, Cahors, France aut Guérin F. Service de Microbiologie & CNR de la Résistance aux Antibiotiques, CHU de Caen, Caen, France aut Lienhard R. ADMED Microbiologie, La Chaux-de-Fonds, Switzerland aut Le Coustumier A. Laboratoire de Biologie, CH de Cahors, Cahors, France aut Daurel C. Service de Microbiologie & CNR de la Résistance aux Antibiotiques, CHU de Caen, Caen, France aut Isnard C. Service de Microbiologie & CNR de la Résistance aux Antibiotiques, CHU de Caen, Caen, France aut Marty N. Service de Bactériologie-Hygiène, CHU de Toulouse, Toulouse, France aut Poyart C. Service de Bactériologie & CNR des Streptocoques, GH Cochin-Hôtel Dieu-Broca, Assistance Publique-Hôpitaux de Paris, Paris, France aut Cattoir V. Service de Microbiologie & CNR de la Résistance aux Antibiotiques, CHU de Caen, Caen, France aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/10(2015-10-01), 2057-2061 0934-9723 34:10<2057 2015 34 10096 https://doi.org/10.1007/s10096-015-2451-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2451-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vergne A. Laboratoire de Biologie, CH de Cahors, Cahors, France aut NATIONALLICENCE 700 1- Guérin F. Service de Microbiologie & CNR de la Résistance aux Antibiotiques, CHU de Caen, Caen, France aut NATIONALLICENCE 700 1- Lienhard R. ADMED Microbiologie, La Chaux-de-Fonds, Switzerland aut NATIONALLICENCE 700 1- Le Coustumier A. Laboratoire de Biologie, CH de Cahors, Cahors, France aut NATIONALLICENCE 700 1- Daurel C. Service de Microbiologie & CNR de la Résistance aux Antibiotiques, CHU de Caen, Caen, France aut NATIONALLICENCE 700 1- Isnard C. Service de Microbiologie & CNR de la Résistance aux Antibiotiques, CHU de Caen, Caen, France aut NATIONALLICENCE 700 1- Marty N. Service de Bactériologie-Hygiène, CHU de Toulouse, Toulouse, France aut NATIONALLICENCE 700 1- Poyart C. Service de Bactériologie & CNR des Streptocoques, GH Cochin-Hôtel Dieu-Broca, Assistance Publique-Hôpitaux de Paris, Paris, France aut NATIONALLICENCE 700 1- Cattoir V. Service de Microbiologie & CNR de la Résistance aux Antibiotiques, CHU de Caen, Caen, France aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/10(2015-10-01), 2057-2061 0934-9723 34:10<2057 2015 34 10096