caa a22 4500 606156976 CHVBK 20210128100611.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10096-014-2291-8 doi (NATIONALLICENCE)springer-10.1007/s10096-014-2291-8 Virulence factors among Staphylococcus lugdunensis are associated with infection sites and clonal spread [Elektronische Daten] [N. Giormezis, F. Kolonitsiou, A. Makri, A. Vogiatzi, M. Christofidou, E. Anastassiou, I. Spiliopoulou] Staphylococcus lugdunensis has emerged as a significant human pathogen, with distinct clinical and microbiological characteristics. Our goal was to identify the virulence factors in S. lugdunensis recovered from infected patients of two Greek hospitals during a six-year period (2008-2013). A collection of 38 S. lugdunensis was tested for biofilm formation, antimicrobial susceptibility, clonal distribution, virulence factors (ica operon, fbl, atlL, vwbl, slush) and antibiotic resistance genes (mecA, ermC) carriage. Strains were classified into pulsotypes by pulsed-field gel electrophoresis (PFGE) of SmaI DNA digests. The majority (22) was isolated from skin and soft tissue infections (SSTIs), nine from deep-sited infections (DSIs), including three bacteraemias and seven from prosthetic device-associated infections (PDAIs). All isolates were oxacillin-susceptible, mecA-negative and fbl-positive. The highest resistance rate was detected for ampicillin (50%), followed by erythromycin and clindamycin (18.4%). Fourteen isolates (36.8%) produced biofilm, whereas 26/38 (68.4%) carried the ica operon. Biofilm formation was more frequent in isolates from PDAIs. Thirty-six strains (94.7%) carried atlL and 31 (81.6%) carried vwbl, whereas slush was detected in 15 (39.5%). PFGE revealed a low level of genetic diversity: strains were classified into seven pulsotypes, with two major clones (C: 22 and D: nine strains). Type C strains recovered from all infection sites prevailed in biofilm formation and ermC carriage, whereas type D strains associated with SSTIs and DSIs carried more frequently vwbl, slush or both genes. Despite susceptibility to antimicrobials, the clonal expansion and carriage of virulence factors, combined with biofilm-producing ability, render this species an important pathogen that should not be ignored. Springer-Verlag Berlin Heidelberg, 2014 Giormezis N. Department of Microbiology, School of Medicine, University of Patras, 26504, Rion, Patras, Greece aut Kolonitsiou F. Department of Microbiology, School of Medicine, University of Patras, 26504, Rion, Patras, Greece aut Makri A. Laboratory of Microbiology, General Children Hospital Pentelis, Athens, Greece aut Vogiatzi A. Laboratory of Microbiology, General Children Hospital Pentelis, Athens, Greece aut Christofidou M. Department of Microbiology, School of Medicine, University of Patras, 26504, Rion, Patras, Greece aut Anastassiou E. Department of Microbiology, School of Medicine, University of Patras, 26504, Rion, Patras, Greece aut Spiliopoulou I. Department of Microbiology, School of Medicine, University of Patras, 26504, Rion, Patras, Greece aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/4(2015-04-01), 773-778 0934-9723 34:4<773 2015 34 10096 https://doi.org/10.1007/s10096-014-2291-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2291-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Giormezis N. Department of Microbiology, School of Medicine, University of Patras, 26504, Rion, Patras, Greece aut NATIONALLICENCE 700 1- Kolonitsiou F. Department of Microbiology, School of Medicine, University of Patras, 26504, Rion, Patras, Greece aut NATIONALLICENCE 700 1- Makri A. Laboratory of Microbiology, General Children Hospital Pentelis, Athens, Greece aut NATIONALLICENCE 700 1- Vogiatzi A. Laboratory of Microbiology, General Children Hospital Pentelis, Athens, Greece aut NATIONALLICENCE 700 1- Christofidou M. Department of Microbiology, School of Medicine, University of Patras, 26504, Rion, Patras, Greece aut NATIONALLICENCE 700 1- Anastassiou E. Department of Microbiology, School of Medicine, University of Patras, 26504, Rion, Patras, Greece aut NATIONALLICENCE 700 1- Spiliopoulou I. Department of Microbiology, School of Medicine, University of Patras, 26504, Rion, Patras, Greece aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/4(2015-04-01), 773-778 0934-9723 34:4<773 2015 34 10096