caa a22 4500 606157069 CHVBK 20210128100611.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10096-014-2300-y doi (NATIONALLICENCE)springer-10.1007/s10096-014-2300-y Staphylococcus pseudintermedius can be misdiagnosed as Staphylococcus aureus in humans with dog bite wounds [Elektronische Daten] [S. Börjesson, E. Gómez-Sanz, K. Ekström, C. Torres, U. Grönlund] The purpose of this study was to investigate whether S. pseudintermedius is misdiagnosed as S. aureus by clinical laboratories when isolated fromhumans with dog bite wounds. In addition, we attempted to determine whether S. pseudintermedius isolates related to dog bite wounds share phenotypic and genotypic traits. S. pseudintermedius was identified by PCR targeting the nuc gene. Isolates were tested for antibiotic susceptibility using VetMIC GP-mo microdilution panels. The occurrence of genes encoding leukocidins, exfoliatins, pyrogenic toxin superantigens and enterotoxins was determined by PCR. The relatedness of S. pseudintermedius isolates was investigated using Multi Locus Sequence Typing (MLST). Out of 101 isolates defined as S. aureus by human clinical microbiology laboratories, 13 isolates were re-identified as S. pseudintermedius and one isolate was confirmed to carry the mecA gene, i.e. methicillin-resistant (MRSP). The MRSP isolate was also defined as multi-resistant. Two methicillin-susceptible S. pseudintermedius isolates were also multi-resistant and five were susceptible to all antibiotics tested. With the exception of three S. pseudintermedius isolates belonging to multi locus sequence type (MLST) 158, all the isolates belonged to unique STs. All isolates contained lukS/F-I, siet and se-int, and expA were identified in two isolates and expB and sec canine-sel in one isolate respectively. S. pseudintermedius is frequently misdiagnosed as S. aureus from humans with dog bite wounds showing that it can act as an opportunistic pathogen in humans. No common phenotypic and genotypic traits shared by the S. pseudintermedius isolates could be identified. Springer-Verlag Berlin Heidelberg, 2014 Börjesson S. Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), SE751 89, Uppsala, Sweden aut Gómez-Sanz E. Department of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain aut Ekström K. Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), SE751 89, Uppsala, Sweden aut Torres C. Department of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain aut Grönlund U. Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), SE751 89, Uppsala, Sweden aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/4(2015-04-01), 839-844 0934-9723 34:4<839 2015 34 10096 https://doi.org/10.1007/s10096-014-2300-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2300-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Börjesson S. Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), SE751 89, Uppsala, Sweden aut NATIONALLICENCE 700 1- Gómez-Sanz E. Department of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain aut NATIONALLICENCE 700 1- Ekström K. Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), SE751 89, Uppsala, Sweden aut NATIONALLICENCE 700 1- Torres C. Department of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain aut NATIONALLICENCE 700 1- Grönlund U. Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), SE751 89, Uppsala, Sweden aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/4(2015-04-01), 839-844 0934-9723 34:4<839 2015 34 10096