caa a22 4500 606157190 CHVBK 20210128100612.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10096-015-2385-y doi (NATIONALLICENCE)springer-10.1007/s10096-015-2385-y Detection of antibody responses against Streptococcus pneumoniae , Haemophilus influenzae , and Moraxella catarrhalis proteins in children with community-acquired pneumonia: effects of combining pneumococcal antigens, pre-existing antibody levels, sampling interval, age, and duration of illness [Elektronische Daten] [I. Borges, D. Andrade, A.-L. Vilas-Boas, M.-S. Fontoura, H. Laitinen, N. Ekström, P. Adrian, A. Meinke, M.-R. Cardoso, A. Barral, O. Ruuskanen, H. Käyhty, C. Nascimento-Carvalho] We evaluated the effects of combining different numbers of pneumococcal antigens, pre-existing antibody levels, sampling interval, age, and duration of illness on the detection of IgG responses against eight Streptococcus pneumoniae proteins, three Haemophilus influenzae proteins, and five Moraxella catarrhalis proteins in 690 children aged <5years with pneumonia. Serological tests were performed on acute and convalescent serum samples with a multiplexed bead-based immunoassay. The median sampling interval was 19days, the median age was 26.7months, and the median duration of illness was 5days. The rate of antibody responses was 15.4% for at least one pneumococcal antigen, 5.8% for H. influenzae, and 2.3% for M. catarrhalis. The rate of antibody responses against each pneumococcal antigen varied from 3.5 to 7.1%. By multivariate analysis, pre-existing antibody levels showed a negative association with the detection of antibody responses against pneumococcal and H. influenzae antigens; the sampling interval was positively associated with the detection of antibody responses against pneumococcal and H. influenzae antigens. A sampling interval of 3 weeks was the optimal cut-off for the detection of antibody responses against pneumococcal and H. influenzae proteins. Duration of illness was negatively associated with antibody responses against PspA. Age did not influence antibody responses against the investigated antigens. In conclusion, serological assays using combinations of different pneumococcal proteins detect a higher rate of antibody responses against S. pneumoniae compared to assays using a single pneumococcal protein. Pre-existing antibody levels and sampling interval influence the detection of antibody responses against pneumococcal and H. influenzae proteins. These factors should be considered when determining pneumonia etiology by serological methods in children. Springer-Verlag Berlin Heidelberg, 2015 Borges I. Postgraduate Programme in Health Sciences, Federal University of Bahia School of Medicine, Praça XV de Novembro, s/n - Largo do Terreiro de Jesus, 40025-010, Salvador, Bahia, Brazil aut Andrade D. Postgraduate Programme in Health Sciences, Federal University of Bahia School of Medicine, Praça XV de Novembro, s/n - Largo do Terreiro de Jesus, 40025-010, Salvador, Bahia, Brazil aut Vilas-Boas A.-L Postgraduate Programme in Health Sciences, Federal University of Bahia School of Medicine, Praça XV de Novembro, s/n - Largo do Terreiro de Jesus, 40025-010, Salvador, Bahia, Brazil aut Fontoura M.-S Department of Paediatrics, Federal University of Bahia School of Medicine, Salvador, Bahia, Brazil aut Laitinen H. National Institute for Health and Welfare, Helsinki, Finland aut Ekström N. National Institute for Health and Welfare, Helsinki, Finland aut Adrian P. DST/NRF Vaccine Preventable Diseases, MRC Respiratory and Meningeal Pathogens Research Unit, University of the Witwatersrand, Johannesburg, South Africa aut Meinke A. Valneva Austria GmbH, Campus Vienna Biocenter 3, Vienna, Austria aut Cardoso M.-R Department of Epidemiology, University of São Paulo School of Public Health, São Paulo, Brazil aut Barral A. Postgraduate Programme in Health Sciences, Federal University of Bahia School of Medicine, Praça XV de Novembro, s/n - Largo do Terreiro de Jesus, 40025-010, Salvador, Bahia, Brazil aut Ruuskanen O. Department of Paediatrics, Turku University and University Hospital, Turku, Finland aut Käyhty H. National Institute for Health and Welfare, Helsinki, Finland aut Nascimento-Carvalho C. Postgraduate Programme in Health Sciences, Federal University of Bahia School of Medicine, Praça XV de Novembro, s/n - Largo do Terreiro de Jesus, 40025-010, Salvador, Bahia, Brazil aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/8(2015-08-01), 1551-1557 0934-9723 34:8<1551 2015 34 10096 https://doi.org/10.1007/s10096-015-2385-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2385-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Borges I. Postgraduate Programme in Health Sciences, Federal University of Bahia School of Medicine, Praça XV de Novembro, s/n - Largo do Terreiro de Jesus, 40025-010, Salvador, Bahia, Brazil aut NATIONALLICENCE 700 1- Andrade D. Postgraduate Programme in Health Sciences, Federal University of Bahia School of Medicine, Praça XV de Novembro, s/n - Largo do Terreiro de Jesus, 40025-010, Salvador, Bahia, Brazil aut NATIONALLICENCE 700 1- Vilas-Boas A.-L Postgraduate Programme in Health Sciences, Federal University of Bahia School of Medicine, Praça XV de Novembro, s/n - Largo do Terreiro de Jesus, 40025-010, Salvador, Bahia, Brazil aut NATIONALLICENCE 700 1- Fontoura M.-S Department of Paediatrics, Federal University of Bahia School of Medicine, Salvador, Bahia, Brazil aut NATIONALLICENCE 700 1- Laitinen H. National Institute for Health and Welfare, Helsinki, Finland aut NATIONALLICENCE 700 1- Ekström N. National Institute for Health and Welfare, Helsinki, Finland aut NATIONALLICENCE 700 1- Adrian P. DST/NRF Vaccine Preventable Diseases, MRC Respiratory and Meningeal Pathogens Research Unit, University of the Witwatersrand, Johannesburg, South Africa aut NATIONALLICENCE 700 1- Meinke A. Valneva Austria GmbH, Campus Vienna Biocenter 3, Vienna, Austria aut NATIONALLICENCE 700 1- Cardoso M.-R Department of Epidemiology, University of São Paulo School of Public Health, São Paulo, Brazil aut NATIONALLICENCE 700 1- Barral A. Postgraduate Programme in Health Sciences, Federal University of Bahia School of Medicine, Praça XV de Novembro, s/n - Largo do Terreiro de Jesus, 40025-010, Salvador, Bahia, Brazil aut NATIONALLICENCE 700 1- Ruuskanen O. Department of Paediatrics, Turku University and University Hospital, Turku, Finland aut NATIONALLICENCE 700 1- Käyhty H. National Institute for Health and Welfare, Helsinki, Finland aut NATIONALLICENCE 700 1- Nascimento-Carvalho C. Postgraduate Programme in Health Sciences, Federal University of Bahia School of Medicine, Praça XV de Novembro, s/n - Largo do Terreiro de Jesus, 40025-010, Salvador, Bahia, Brazil aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/8(2015-08-01), 1551-1557 0934-9723 34:8<1551 2015 34 10096