caa a22 4500 606157239 CHVBK 20210128100612.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10096-015-2383-0 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2383-0 Performance of Gram staining on blood cultures flagged negative by an automated blood culture system [Elektronische Daten] [A. Peretz, N. Isakovich, N. Pastukh, A. Koifman, T. Glyatman, D. Brodsky] Blood is one of the most important specimens sent to a microbiology laboratory for culture. Most blood cultures are incubated for 5-7days, except in cases where there is a suspicion of infection caused by microorganisms that proliferate slowly, or infections expressed by a small number of bacteria in the bloodstream. Therefore, at the end of incubation, misidentification of positive cultures and false-negative results are a real possibility. The aim of this work was to perform a confirmation by Gram staining of the lack of any microorganisms in blood cultures that were identified as negative by the BACTEC™ FX system at the end of incubation. All bottles defined as negative by the BACTEC FX system were Gram-stained using an automatic device and inoculated on solid growth media. In our work, 15 cultures that were defined as negative by the BACTEC FX system at the end of the incubation were found to contain microorganisms when Gram-stained. The main characteristic of most bacteria and fungi growing in the culture bottles that were defined as negative was slow growth. This finding raises a problematic issue concerning the need to perform Gram staining of all blood cultures, which could overload the routine laboratory work, especially laboratories serving large medical centers and receiving a large number of blood cultures. Springer-Verlag Berlin Heidelberg, 2015 Peretz A. Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Affiliated with the Faculty of Medicine, Bar-Ilan University, Galilee, Israel aut Isakovich N. Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Affiliated with the Faculty of Medicine, Bar-Ilan University, Galilee, Israel aut Pastukh N. Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Affiliated with the Faculty of Medicine, Bar-Ilan University, Galilee, Israel aut Koifman A. Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Affiliated with the Faculty of Medicine, Bar-Ilan University, Galilee, Israel aut Glyatman T. Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Affiliated with the Faculty of Medicine, Bar-Ilan University, Galilee, Israel aut Brodsky D. Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Affiliated with the Faculty of Medicine, Bar-Ilan University, Galilee, Israel aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/8(2015-08-01), 1539-1541 0934-9723 34:8<1539 2015 34 10096 https://doi.org/10.1007/s10096-015-2383-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2383-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peretz A. Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Affiliated with the Faculty of Medicine, Bar-Ilan University, Galilee, Israel aut NATIONALLICENCE 700 1- Isakovich N. Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Affiliated with the Faculty of Medicine, Bar-Ilan University, Galilee, Israel aut NATIONALLICENCE 700 1- Pastukh N. Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Affiliated with the Faculty of Medicine, Bar-Ilan University, Galilee, Israel aut NATIONALLICENCE 700 1- Koifman A. Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Affiliated with the Faculty of Medicine, Bar-Ilan University, Galilee, Israel aut NATIONALLICENCE 700 1- Glyatman T. Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Affiliated with the Faculty of Medicine, Bar-Ilan University, Galilee, Israel aut NATIONALLICENCE 700 1- Brodsky D. Clinical Microbiology Laboratory, Baruch Padeh Medical Center, Poriya, Affiliated with the Faculty of Medicine, Bar-Ilan University, Galilee, Israel aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/8(2015-08-01), 1539-1541 0934-9723 34:8<1539 2015 34 10096