caa a22 4500 606157247 CHVBK 20210128100612.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10096-015-2381-2 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2381-2 Implementation of MALDI-TOF MS technology for the identification of clinical isolates of Mycobacterium spp. in mycobacterial diagnosis [Elektronische Daten] [G. Tudó, M. Monté, A. Vergara, A. López, J. Hurtado, M. Ferrer-Navarro, J. Vila, J. Gonzalez-Martin] A total of 243 clinical isolates of the Mycobacterium genus were studied, 143 and 100 using two protocols (Protocol v2 and Protocol v3, respectively) provided by the manufacturer. The overall correlation of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with the standard identification methods was 63.8%. The rate of misidentification was 3.2%, mainly affecting very close species. In Protocol v2, the correlation was 57.3%, being greater in solid than in liquid media (71.7% vs. 44.7%, p < 0.05). Albeit not significant, a trend to a greater correlation for M. tuberculosis complex compared to non-tuberculous mycobacteria (NTM) (63.6% vs. 55.5%) was observed. In Protocol v3, the correlation was 73%, with no significant differences between solid and liquid media (70.8% vs. 75%). In conclusion, MALDI-TOF MS may play a role in identifying mycobacterial species isolated from clinical samples, being faster than sequencing and hybridization-based techniques. Springer-Verlag Berlin Heidelberg, 2015 Tudó G. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut Monté M. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut Vergara A. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut López A. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut Hurtado J. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut Ferrer-Navarro M. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut Vila J. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut Gonzalez-Martin J. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/8(2015-08-01), 1527-1532 0934-9723 34:8<1527 2015 34 10096 https://doi.org/10.1007/s10096-015-2381-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2381-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tudó G. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut NATIONALLICENCE 700 1- Monté M. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut NATIONALLICENCE 700 1- Vergara A. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut NATIONALLICENCE 700 1- López A. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut NATIONALLICENCE 700 1- Hurtado J. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut NATIONALLICENCE 700 1- Ferrer-Navarro M. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut NATIONALLICENCE 700 1- Vila J. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut NATIONALLICENCE 700 1- Gonzalez-Martin J. Servei de Microbiologia, CDB, Hospital Clínic de Barcelona—Institut de Salut Global de Barcelona (ISGlobal), Universitat de Barcelona, c/ Villarroel 170, 08036, Barcelona, Spain aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/8(2015-08-01), 1527-1532 0934-9723 34:8<1527 2015 34 10096