caa a22 4500 606157263 CHVBK 20210128100612.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10096-015-2389-7 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2389-7 Giardia duodenalis assemblages in Egyptian children with diarrhea [Elektronische Daten] [H. Fahmy, A. El-Serougi, H. El Deeb, H. Hussein, H. Abou-Seri, C. Klotz, T. Aebischer, K. El Sayed Khalifa Mohamed] Giardia duodenalis is considered the most common cause of parasitic diarrhea worldwide. Genetic studies revealed that at least eight assemblages (A-H) exist. Of these assemblages, A and B are found primarily in human beings and occasionally in animals. The association between clinical symptoms and G. duodenalis assemblages is controversial. The aim of the present study was to determine the assemblages of G. duodenalis prevalent among Egyptian children with diarrhea. Therefore, 96 positive stool samples for Giardia by light microscopy were subjected to multilocus genotyping targeting the triose phosphate isomerase (tpi), β-giardin (bg), and glutamate dehydrogenase (gdh) genes. Amplified polymerase chain reaction (PCR) products were then purified, sequenced, and aligned with reference strains to determine the assemblages of the Giardia isolates. Out of the 96 microscopically positive stool samples for Giardia, 77 (80%) were successfully amplified and sequenced at least at one locus. Of these, 21 (27.3%) were shown to be assemblage A, 54 (70.1%) assemblage B, while discordant sequence typing results were observed in 2 (2.6%) samples. AII was the predominant subassemblage of assemblage A, while it was generally difficult to further classify assemblage B. It was concluded that infection with assemblage B was more common than that with assemblage A. No associations between epidemiological information and assemblage were detected, except with age. Although infections with assemblage B were more frequently associated with abdominal pain and acute diarrhea than with assemblage A, the difference was not statistically significant. Springer-Verlag Berlin Heidelberg, 2015 Fahmy H. Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut El-Serougi A. Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut El Deeb H. Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut Hussein H. Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut Abou-Seri H. Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut Klotz C. Mycotic and Parasitic Agents and Mycobacteria, Department of Infectious Diseases, Robert Koch-Institute, 13353, Berlin, Germany aut Aebischer T. Mycotic and Parasitic Agents and Mycobacteria, Department of Infectious Diseases, Robert Koch-Institute, 13353, Berlin, Germany aut El Sayed Khalifa Mohamed K. Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/8(2015-08-01), 1573-1581 0934-9723 34:8<1573 2015 34 10096 https://doi.org/10.1007/s10096-015-2389-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2389-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fahmy H. Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- El-Serougi A. Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- El Deeb H. Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- Hussein H. Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- Abou-Seri H. Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- Klotz C. Mycotic and Parasitic Agents and Mycobacteria, Department of Infectious Diseases, Robert Koch-Institute, 13353, Berlin, Germany aut NATIONALLICENCE 700 1- Aebischer T. Mycotic and Parasitic Agents and Mycobacteria, Department of Infectious Diseases, Robert Koch-Institute, 13353, Berlin, Germany aut NATIONALLICENCE 700 1- El Sayed Khalifa Mohamed K. Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/8(2015-08-01), 1573-1581 0934-9723 34:8<1573 2015 34 10096