caa a22 4500 606157271 CHVBK 20210128100612.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10096-015-2387-9 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2387-9 Staphylococcus aureus nasal carriage among healthcare workers in Kisangani, the Democratic Republic of the Congo [Elektronische Daten] [H. De Boeck, S. Vandendriessche, M. Hallin, B. Batoko, J.-P. Alworonga, B. Mapendo, C. Van Geet, N. Dauly, O. Denis, J. Jacobs] Methicillin-resistant Staphylococcus aureus (MRSA) is a global health concern, but there are few data from Central Africa. The objective of our study was to characterise S. aureus colonisation isolates from healthcare-exposed professionals in the Democratic Republic of the Congo (DRC). Healthcare workers and medical students (n = 380) in Kisangani, DRC were screened for S. aureus nasal carriage in a single-centre cross-sectional study in the University Hospital of Kisangani. The isolates were identified and characterised using phenotypic and genotypic methods. The nasal carriage rate of S. aureus was 16.6% and 10 out of 63 isolates (15.9%) were MRSA. We found 28 different spa types. Most MRSA isolates belonged to ST8-spa t1476-SCCmec V. The majority of MRSA were multidrug-resistant to non-beta-lactam antibiotics. Overall, 28.5% of S. aureus carried Panton-Valentine leucocidin (PVL)-encoding genes (all methicillin-sensitive) and 17.5% carried toxic shock syndrome toxin-1 (TSST-1)-encoding genes. The finding of MRSA carriage among healthcare workers in a setting with limited access to diagnostic microbiology and appropriate therapy calls for improved education on infection control practices and supports the introduction of surveillance programmes. Springer-Verlag Berlin Heidelberg, 2015 De Boeck H. Department of Clinical Sciences, Institute of Tropical Medicine (ITM), Nationalestraat 155, 2000, Antwerp, Belgium aut Vandendriessche S. Centre National de Référence MRSA Staphylocoques, Microbiology, Hôpital Erasme—Université Livre de Bruxelles, Lennikse Baan 808, 1070, Anderlecht, Belgium aut Hallin M. Centre National de Référence MRSA Staphylocoques, Microbiology, Hôpital Erasme—Université Livre de Bruxelles, Lennikse Baan 808, 1070, Anderlecht, Belgium aut Batoko B. Department of Pediatrics, University Hospital Kisangani, Kisangani, Democratic Republic of the Congo aut Alworonga J.-P Department of Pediatrics, University Hospital Kisangani, Kisangani, Democratic Republic of the Congo aut Mapendo B. Department of Pediatrics, University Hospital Kisangani, Kisangani, Democratic Republic of the Congo aut Van Geet C. Department of Pediatrics, KU Leuven, Herestraat 49, 3000, Leuven, Belgium aut Dauly N. Department of Pediatrics, University Hospital Kisangani, Kisangani, Democratic Republic of the Congo aut Denis O. Centre National de Référence MRSA Staphylocoques, Microbiology, Hôpital Erasme—Université Livre de Bruxelles, Lennikse Baan 808, 1070, Anderlecht, Belgium aut Jacobs J. Department of Clinical Sciences, Institute of Tropical Medicine (ITM), Nationalestraat 155, 2000, Antwerp, Belgium aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/8(2015-08-01), 1567-1572 0934-9723 34:8<1567 2015 34 10096 https://doi.org/10.1007/s10096-015-2387-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2387-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Boeck H. Department of Clinical Sciences, Institute of Tropical Medicine (ITM), Nationalestraat 155, 2000, Antwerp, Belgium aut NATIONALLICENCE 700 1- Vandendriessche S. Centre National de Référence MRSA Staphylocoques, Microbiology, Hôpital Erasme—Université Livre de Bruxelles, Lennikse Baan 808, 1070, Anderlecht, Belgium aut NATIONALLICENCE 700 1- Hallin M. Centre National de Référence MRSA Staphylocoques, Microbiology, Hôpital Erasme—Université Livre de Bruxelles, Lennikse Baan 808, 1070, Anderlecht, Belgium aut NATIONALLICENCE 700 1- Batoko B. Department of Pediatrics, University Hospital Kisangani, Kisangani, Democratic Republic of the Congo aut NATIONALLICENCE 700 1- Alworonga J.-P Department of Pediatrics, University Hospital Kisangani, Kisangani, Democratic Republic of the Congo aut NATIONALLICENCE 700 1- Mapendo B. Department of Pediatrics, University Hospital Kisangani, Kisangani, Democratic Republic of the Congo aut NATIONALLICENCE 700 1- Van Geet C. Department of Pediatrics, KU Leuven, Herestraat 49, 3000, Leuven, Belgium aut NATIONALLICENCE 700 1- Dauly N. Department of Pediatrics, University Hospital Kisangani, Kisangani, Democratic Republic of the Congo aut NATIONALLICENCE 700 1- Denis O. Centre National de Référence MRSA Staphylocoques, Microbiology, Hôpital Erasme—Université Livre de Bruxelles, Lennikse Baan 808, 1070, Anderlecht, Belgium aut NATIONALLICENCE 700 1- Jacobs J. Department of Clinical Sciences, Institute of Tropical Medicine (ITM), Nationalestraat 155, 2000, Antwerp, Belgium aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/8(2015-08-01), 1567-1572 0934-9723 34:8<1567 2015 34 10096