caa a22 4500 606157484 CHVBK 20210128100614.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10096-015-2312-2 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2312-2 Low prevalence of Blastocystis sp. in active ulcerative colitis patients [Elektronische Daten] [N. Rossen, A. Bart, N. Verhaar, E. van Nood, R. Kootte, P. de Groot, G. D'Haens, C. Ponsioen, T. van Gool] Ulcerative colitis (UC) is thought to originate from a disbalance in the interplay between the gut microbiota and the innate and adaptive immune system. Apart from the bacterial microbiota, there might be other organisms, such as parasites or viruses, that could play a role in the aetiology of UC. The primary objective of this study was to compare the prevalence of Blastocystis sp. in a cohort of patients with active UC and compare that to the prevalence in healthy controls. We studied patients with active UC confirmed by endoscopy included in a randomised prospective trial on the faecal transplantation for UC. A cohort of healthy subjects who served as donors in randomised trials on faecal transplantation were controls. Healthy subjects did not have gastrointestinal symptoms and were extensively screened for infectious diseases by a screenings questionnaire, extensive serologic assessment for viruses and stool analysis. Potential parasitic infections such as Blastocystis were diagnosed with the triple faeces test (TFT). The prevalence of Blastocystis sp. were compared between groups by Chi-square testing. A total of 168 subjects were included, of whom 45 had active UC [median age 39.0years, interquartile range (IQR) 32.5-49.0, 49% male] and 123 were healthy subjects (median age 27years, IQR 22.0-37.0, 54% male). Blastocystis sp. was present in the faeces of 40/123 (32.5%) healthy subjects and 6/45 (13.3%) UC patients (p = 0.014). Infection with Blastocystis is significantly less frequent in UC patients as compared to healthy controls. The Author(s), 2015 Rossen N. Department of Gastroenterology and Hepatology, Academic Medical Center, Amsterdam, The Netherlands aut Bart A. Department of Medical Microbiology, Parasitology Section, Academic Medical Center, Amsterdam, The Netherlands aut Verhaar N. Department of Medical Microbiology, Parasitology Section, Academic Medical Center, Amsterdam, The Netherlands aut van Nood E. Department of Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands aut Kootte R. Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands aut de Groot P. Department of Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands aut D'Haens G. Department of Gastroenterology and Hepatology, Academic Medical Center, Amsterdam, The Netherlands aut Ponsioen C. Department of Gastroenterology and Hepatology, Academic Medical Center, Amsterdam, The Netherlands aut van Gool T. Department of Medical Microbiology, Parasitology Section, Academic Medical Center, Amsterdam, The Netherlands aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 1039-1044 0934-9723 34:5<1039 2015 34 10096 https://doi.org/10.1007/s10096-015-2312-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2312-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rossen N. Department of Gastroenterology and Hepatology, Academic Medical Center, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Bart A. Department of Medical Microbiology, Parasitology Section, Academic Medical Center, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Verhaar N. Department of Medical Microbiology, Parasitology Section, Academic Medical Center, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- van Nood E. Department of Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Kootte R. Department of Vascular Medicine, Academic Medical Center, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- de Groot P. Department of Internal Medicine, Academic Medical Center, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- D'Haens G. Department of Gastroenterology and Hepatology, Academic Medical Center, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- Ponsioen C. Department of Gastroenterology and Hepatology, Academic Medical Center, Amsterdam, The Netherlands aut NATIONALLICENCE 700 1- van Gool T. Department of Medical Microbiology, Parasitology Section, Academic Medical Center, Amsterdam, The Netherlands aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 1039-1044 0934-9723 34:5<1039 2015 34 10096