caa a22 4500 606157506 CHVBK 20210128100614.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10096-015-2321-1 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2321-1 First isolation of Coxiella burnetii from clinical material by cell-free medium (ACCM2) [Elektronische Daten] [K. Boden, K. Wolf, B. Hermann, D. Frangoulidis] A disadvantage in Q fever diagnostics and research is the insensitive and difficult culture of Coxiella burnetii. This intracellular organism can only be isolated using embryonated eggs, animal hosts, or mammalian cell culture. In consequence, it has only been possible to isolate a few strains from human patients. Here, we describe the first isolation of C. burnetii from a clinical specimen using the recently developed cell-free medium acidified citrate cysteine medium 2 (ACCM2). We screened the sera of 217 patients who had undergone valvular transplantation but detected one serum with an antibody constellation indicating chronic Q fever. Polymerase chain reaction (PCR) of the corresponding heart valve revealed 3.1 × 105 copies/rxn. The strain was successfully isolated using ACCM2. Genomic investigation by multilocus variable-number of tandem repeat (VNTR) analysis (MLVA) revealed the strain to be a new genotype, A10, closely related to one from sheep. As the sensitivity of ACCM2 for different human strains is unknown, we also investigated combining a robust test, egg propagation, with ACCM2. This combination produced four to six logs of growth of the bacteria. The use of ACCM2 in this combination simplified the otherwise elaborate purification steps. Cultivation in ACCM2 has the potential to simplify the isolation of C. burnetii in a clinical setting. As the success rates of cell culture for virulent C. burnetii strains are variable, the sensitivity of ACCM2 for different strains is unknown, and many specimens may contain much fewer bacteria than in our case, the combination of the robust method of egg propagation with ACCM2 is a good alternative to existing single methods for investigating critical specimens. Springer-Verlag Berlin Heidelberg, 2015 Boden K. University Hospital Jena, Jena, Germany aut Wolf K. University Hospital Jena, Jena, Germany aut Hermann B. University Hospital Jena, Jena, Germany aut Frangoulidis D. Bundeswehr Institute of Microbiology, Munich, Germany aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 1017-1022 0934-9723 34:5<1017 2015 34 10096 https://doi.org/10.1007/s10096-015-2321-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2321-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boden K. University Hospital Jena, Jena, Germany aut NATIONALLICENCE 700 1- Wolf K. University Hospital Jena, Jena, Germany aut NATIONALLICENCE 700 1- Hermann B. University Hospital Jena, Jena, Germany aut NATIONALLICENCE 700 1- Frangoulidis D. Bundeswehr Institute of Microbiology, Munich, Germany aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 1017-1022 0934-9723 34:5<1017 2015 34 10096