caa a22 4500 606157549 CHVBK 20210128100614.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10096-014-2298-1 doi (NATIONALLICENCE)springer-10.1007/s10096-014-2298-1 Faecal carriage of carbapenemase-producing Gram-negative bacilli in hospital settings in southern France [Elektronische Daten] [A. Pantel, H. Marchandin, M.-F. Prère, A. Boutet-Dubois, N. Brieu-Roche, A. Gaschet, A. Davin-Regli, A. Sotto, J.-P. Lavigne] The emergence of carbapenemase-producing Gram-negative bacilli is a worldwide problem. To date, no study has evaluated the prevalence of faecal carriage of carbapenemase-producing and carbapenem-resistant Gram-negative bacilli (CR GNB) in France. From 1 February to 30 April 2012, we conducted a prospective, multicentre study in three University Hospitals and four General Hospitals in the south of France. The carriage of carbapenemase-producing Enterobacteriaceae (CPE) and other CR GNB was screened by both cultivation on chromID® CARBA and chromID® OXA-48 media (bioMérieux) and molecular tools [multiplex polymerase chain reaction (PCR) and NucliSENS EasyQ® KPC (bioMérieux)]. The genetic relationship between isolates was assessed by rep-PCR (DiversiLab, bioMérieux) or multilocus sequence typing (MLST). The prevalences of CR GNB and carbapenemase-producing bacteria were 2.4% (27/1,135) and 0.4% (n = 5), respectively. Two strains corresponded to OXA-23-producing Acinetobacter baumannii and belonged to the widespread sequence type (ST) 2/international clone II, whereas one strain was an ST15 OXA-48-producing Klebsiella pneumoniae. Two OXA-48-producers were detected exclusively by PCR. This first French study revealed the very low dissemination of carbapenemase-producing bacteria in patients attending hospitals in southern France during a non-outbreak situation. However, the increasing description of epidemic cases in this area must reinforce the use of hygiene procedures to prevent diffusion of these multidrug-resistant microorganisms. Springer-Verlag Berlin Heidelberg, 2014 Pantel A. Service de Microbiologie, CHU Carémeau, Place du Professeur Robert Debré, Nîmes, France aut Marchandin H. Laboratoire de Bactériologie, CHU Arnaud de Villeneuve, Montpellier, France aut Prère M.-F Laboratoire de Bactériologie-Hygiène, CHU Purpan, Toulouse, France aut Boutet-Dubois A. Institut National de la Santé et de la Recherche Médicale, U1047, Université Montpellier 1, Nîmes, France aut Brieu-Roche N. Laboratoire de Biologie Polyvalente, CH du Pays d'Aix, Aix-en-Provence, France aut Gaschet A. Laboratoire de Biologie Polyvalente, CH Saint-Jean, Perpignan, France aut Davin-Regli A. Laboratoire de Biologie Polyvalente, CH Edmond Garcin, Aubagne, France aut Sotto A. Institut National de la Santé et de la Recherche Médicale, U1047, Université Montpellier 1, Nîmes, France aut Lavigne J.-P Service de Microbiologie, CHU Carémeau, Place du Professeur Robert Debré, Nîmes, France aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 899-904 0934-9723 34:5<899 2015 34 10096 https://doi.org/10.1007/s10096-014-2298-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2298-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pantel A. Service de Microbiologie, CHU Carémeau, Place du Professeur Robert Debré, Nîmes, France aut NATIONALLICENCE 700 1- Marchandin H. Laboratoire de Bactériologie, CHU Arnaud de Villeneuve, Montpellier, France aut NATIONALLICENCE 700 1- Prère M.-F Laboratoire de Bactériologie-Hygiène, CHU Purpan, Toulouse, France aut NATIONALLICENCE 700 1- Boutet-Dubois A. Institut National de la Santé et de la Recherche Médicale, U1047, Université Montpellier 1, Nîmes, France aut NATIONALLICENCE 700 1- Brieu-Roche N. Laboratoire de Biologie Polyvalente, CH du Pays d'Aix, Aix-en-Provence, France aut NATIONALLICENCE 700 1- Gaschet A. Laboratoire de Biologie Polyvalente, CH Saint-Jean, Perpignan, France aut NATIONALLICENCE 700 1- Davin-Regli A. Laboratoire de Biologie Polyvalente, CH Edmond Garcin, Aubagne, France aut NATIONALLICENCE 700 1- Sotto A. Institut National de la Santé et de la Recherche Médicale, U1047, Université Montpellier 1, Nîmes, France aut NATIONALLICENCE 700 1- Lavigne J.-P Service de Microbiologie, CHU Carémeau, Place du Professeur Robert Debré, Nîmes, France aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 899-904 0934-9723 34:5<899 2015 34 10096