caa a22 4500 606157581 CHVBK 20210128100614.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10096-015-2318-9 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2318-9 Performance of different culture methods and of a commercial molecular assay for the detection of carbapenemase-producing Enterobacteriaceae in nursing homes and rehabilitation centers [Elektronische Daten] [V. Saegeman, J. Van den Eynde, L. Niclaes, D. De Ridder, A. Schuermans, Y. Glupczynski] Over the last several years, carbapenemase-producing Enterobacteriaceae (CPE) have been increasingly detected not only among patients in acute care hospitals, but also in long-term care facilities. In this point prevalence survey, residents from three nursing homes and patients in one rehabilitation center were screened for asymptomatic intestinal carriage of CPE by rectal swabs. The first objective was to evaluate the hypothesis of the establishment of a CPE reservoir in a geriatric/chronic care population. Secondly, we evaluated the comparative performances of different culture methods (chromID® CARBA, chromID® OXA-48, MacConkey with temocillin/meropenem, ertapenem enrichment broth) and a commercial molecular assay (Check-Direct CPE). From the 257 included residents, only one had evidence for CPE carriage. From the rectal swabs of this resident, an OXA-48-producing Klebsiella pneumoniae could be isolated and was confirmed by a molecular assay both on the strain and on the rectal swab. The specificity of the different culture methods and Check-Direct CPE was at least 97%. Neither enrichment broth nor prolonged incubation up to 48h increased the yield of CPE. This point prevalence survey shows a low CPE prevalence of 0.39%. Larger scaled studies are needed in order to confirm the role of chronic care settings as secondary CPE reservoirs and to adjust the infection control and prevention recommendations. Springer-Verlag Berlin Heidelberg, 2015 Saegeman V. Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium aut Van den Eynde J. Chronic Care Center De Plataan, Hospitaalstraat 12, Sint-Niklaas, Belgium aut Niclaes L. Chronic Care Center Ter Vlierbeke, Rustoordlaan 3, Kessel-Lo, Belgium aut De Ridder D. National Multiple Sclerosis Center, Vanheylenstraat 16, Melsbroek, Belgium aut Schuermans A. Department of Infection Control and Epidemiology, University Hospitals Leuven, Leuven, Belgium aut Glupczynski Y. National Reference Center for Monitoring of Antimicrobial Resistance in Gram-negative Bacteria, CHU Dinant Godinne, Université Catholique de Louvain (UCL), Louvain-la-Neuve, Belgium aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 991-997 0934-9723 34:5<991 2015 34 10096 https://doi.org/10.1007/s10096-015-2318-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2318-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saegeman V. Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium aut NATIONALLICENCE 700 1- Van den Eynde J. Chronic Care Center De Plataan, Hospitaalstraat 12, Sint-Niklaas, Belgium aut NATIONALLICENCE 700 1- Niclaes L. Chronic Care Center Ter Vlierbeke, Rustoordlaan 3, Kessel-Lo, Belgium aut NATIONALLICENCE 700 1- De Ridder D. National Multiple Sclerosis Center, Vanheylenstraat 16, Melsbroek, Belgium aut NATIONALLICENCE 700 1- Schuermans A. Department of Infection Control and Epidemiology, University Hospitals Leuven, Leuven, Belgium aut NATIONALLICENCE 700 1- Glupczynski Y. National Reference Center for Monitoring of Antimicrobial Resistance in Gram-negative Bacteria, CHU Dinant Godinne, Université Catholique de Louvain (UCL), Louvain-la-Neuve, Belgium aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 991-997 0934-9723 34:5<991 2015 34 10096