caa a22 4500 606157611 CHVBK 20210128100614.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10096-014-2305-6 doi (NATIONALLICENCE)springer-10.1007/s10096-014-2305-6 The MAST® D68C test: an interesting tool for detecting extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae [Elektronische Daten] [C. Nourrisson, R. Tan, C. Hennequin, L. Gibold, R. Bonnet, F. Robin] The Mast® D68C test is a phenotypical test that allows the detection of extended-spectrum β-lactamase (ESBL) production, even in AmpC-producing Enterobacteriaceae. We assessed its detection accuracy against a large collection of 106 Enterobacteriaceae isolates producing a wide diversity of well-characterized β-lactamases (53 ESBL producers, 25 Amp. producers, seven AmpC and ESBL producers, five carbapenemase producers, three carbapenemase and ESBL producers, one AmpC, carbapenemase, and ESBL producer, three TEM-1 producers, three SHV-1 producers, three OXA-1 producers, and one hyperOXY producer, ATCC 35218, ATCC 25922 [a β-lactamase-negative control strain]). The results were compared with those of the double disk test and the Clinical and Laboratory Standards Institute (CLSI) confirmatory test for the detection of ESBL. The sensitivity was 90.6% for the synergy test, 87.5% for the CLSI method, and only 73.1% for D68C, which, however, reached 92.1% if the strains for which supplementary investigations were recommended and the complex mutant TEM (CMT)-producing strains were excluded versus 94.1% and 88.2% for the other methods. The specificity was 90.2% for the synergy test and 100% for the CLSI method and D68C. D68C was also efficient in detecting AmpC-overproducing strains (sensitivity = 97%, specificity = 95.9%): among the 74 strains belonging to natural AmpC-producing species, the sensitivity and specificity were 100 and 94.8%, respectively. The Mast® D68C-test is a promising method that is easy to perform for the detection of current ESBLs and could also be useful for the detection of plasmid-encoded AmpC enzymes (sensitivity = 100%). Springer-Verlag Berlin Heidelberg, 2015 Nourrisson C. Laboratoire de Bactériologie, CHU Clermont-Ferrand, 58, rue Montalembert, 63003, Clermont-Ferrand, France aut Tan R. Laboratoire de Bactériologie, CHU Clermont-Ferrand, 58, rue Montalembert, 63003, Clermont-Ferrand, France aut Hennequin C. Laboratoire de Bactériologie, CHU Clermont-Ferrand, 58, rue Montalembert, 63003, Clermont-Ferrand, France aut Gibold L. Laboratoire de Bactériologie, CHU Clermont-Ferrand, 58, rue Montalembert, 63003, Clermont-Ferrand, France aut Bonnet R. Laboratoire de Bactériologie, CHU Clermont-Ferrand, 58, rue Montalembert, 63003, Clermont-Ferrand, France aut Robin F. Laboratoire de Bactériologie, CHU Clermont-Ferrand, 58, rue Montalembert, 63003, Clermont-Ferrand, France aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 975-983 0934-9723 34:5<975 2015 34 10096 https://doi.org/10.1007/s10096-014-2305-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2305-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nourrisson C. Laboratoire de Bactériologie, CHU Clermont-Ferrand, 58, rue Montalembert, 63003, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Tan R. Laboratoire de Bactériologie, CHU Clermont-Ferrand, 58, rue Montalembert, 63003, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Hennequin C. Laboratoire de Bactériologie, CHU Clermont-Ferrand, 58, rue Montalembert, 63003, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Gibold L. Laboratoire de Bactériologie, CHU Clermont-Ferrand, 58, rue Montalembert, 63003, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Bonnet R. Laboratoire de Bactériologie, CHU Clermont-Ferrand, 58, rue Montalembert, 63003, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Robin F. Laboratoire de Bactériologie, CHU Clermont-Ferrand, 58, rue Montalembert, 63003, Clermont-Ferrand, France aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 975-983 0934-9723 34:5<975 2015 34 10096