caa a22 4500 606157654 CHVBK 20210128100615.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10096-015-2324-y doi (NATIONALLICENCE)springer-10.1007/s10096-015-2324-y Determination of MICING: a new assay for assessing m inimal i nhibitory c oncentration for in vasive g rowth [Elektronische Daten] [J. Zupan, Z. Tomičić, P. Raspor] Our work was focused on a new assay for characterising clinically important yeast. This assay was developed due to the need for new diagnostic methods for recognising potentially virulent strains of increasingly important non-albicans yeast pathogens, such as Saccharomyces cerevisiae and Candida glabrata. With the great diversity among strains for virulence and virulence factors, identification to the species level is not sufficient; therefore, testing for specific virulent traits remains the best option. We show here that the proposed assay uncovers the relationships between the three most important yeast virulence traits in a single test: the ability of a strain to invade solid medium, while resisting the presence of an antimycotic and high temperature (37°C). We combined the quantitative agar invasion assay with classical antimycotic susceptibility testing into a single assay. Similarly to the minimal inhibitory concentration (MIC) value, we defined the MICING (minimal inhibitory concentration of antimycotic for invasive growth) as the concentration of an antimycotic above which the yeast invasive growth is significantly repressed. In this study, we tested three of the most common antimycotics: fluconazole, itraconazole and amphotericin B. The response of yeast strains invasion was characteristic of each antimycotic, indicating their mechanisms of action. In addition to MICING, the assay provides quantitative information about the superficial and invasive growth, and also about the relative invasion, which helps in identifying clinically important yeast, such as azole-resistant and/or invasive strains of S. cerevisiae and C. glabrata. Springer-Verlag Berlin Heidelberg, 2015 Zupan J. Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia aut Tomičić Z. Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000, Novi Sad, Serbia aut Raspor P. Faculty of Health Sciences, University of Primorska, Polje 42, 6310, Izola, Slovenia aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 1023-1030 0934-9723 34:5<1023 2015 34 10096 https://doi.org/10.1007/s10096-015-2324-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2324-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zupan J. Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Tomičić Z. Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000, Novi Sad, Serbia aut NATIONALLICENCE 700 1- Raspor P. Faculty of Health Sciences, University of Primorska, Polje 42, 6310, Izola, Slovenia aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 1023-1030 0934-9723 34:5<1023 2015 34 10096