caa a22 4500 606157700 CHVBK 20210128100615.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10096-015-2317-x doi (NATIONALLICENCE)springer-10.1007/s10096-015-2317-x How to optimise the yield of forensic and clinical post-mortem microbiology with an adequate sampling: a proposal for standardisation [Elektronische Daten] [A. Fernández-Rodríguez, M. Cohen, J. Lucena, W. Van de Voorde, A. Angelini, N. Ziyade, V. Saegeman] Post-mortem microbiology (PMM) is an important tool in forensic pathology, helping to determine the cause and manner of death, especially in difficult scenarios such as sudden unexpected death (SD). Currently, there is a lack of standardization of PMM sampling throughout Europe. We present recommendations elaborated by a panel of European experts aimed to standardize microbiological sampling in the most frequent forensic and clinical post-mortem situations. A network of forensic microbiologists, pathologists and physicians from Spain, England, Belgium, Italy and Turkey shaped a flexible protocol providing minimal requirements for PMM sampling at four practical scenarios: SD, bioterrorism, tissue and cell transplantation (TCT) and paleomicrobiology. Biosafety recommendations were also included. SD was categorized into four subgroups according to the age of the deceased and circumstances at autopsy: (1) included SD in infancy and childhood (0-16 years); (2) corresponded to SD in the young (17-35 years); (3) comprised SD at any age with clinical symptoms; and (4) included traumatic/iatrogenic SD. For each subgroup, a minimum set of samples and general recommendations for microbiological analyses were established. Sampling recommendations for main bioterrorism scenarios were provided. In the TCT setting, the Belgian sampling protocol was presented as an example. Finally, regarding paleomicrobiology, the sampling selection for different types of human remains was reviewed. This proposal for standardization in the sampling constitutes the first step towards a consensus in PMM procedures. In addition, the protocol flexibility to adapt the sampling to the clinical scenario and specific forensic findings adds a cost-benefit value. Springer-Verlag Berlin Heidelberg, 2015 Fernández-Rodríguez A. Forensic Microbiology Laboratory, Instituto Nacional de Toxicología y Ciencias Forenses, Madrid, Spain aut Cohen M. Histopathology Department, Sheffield Children's NHS FT, Sheffield, UK aut Lucena J. Forensic Pathology Service, Institute of Legal Medicine, Seville, Spain aut Van de Voorde W. Department of Forensic Medicine, KU Leuven - University of Leuven, University Hospitals Leuven, 3000, Leuven, Belgium aut Angelini A. Department of Cardiac, Thoracic and Vascular Sciences, University of Padua, Padua, Italy aut Ziyade N. Postmortem Microbiology Laboratory, Council of Forensic Medicine, Istanbul, Turkey aut Saegeman V. Department of Laboratory Medicine, KU Leuven - University Hospitals Leuven, 3000, Leuven, Belgium aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 1045-1057 0934-9723 34:5<1045 2015 34 10096 https://doi.org/10.1007/s10096-015-2317-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2317-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fernández-Rodríguez A. Forensic Microbiology Laboratory, Instituto Nacional de Toxicología y Ciencias Forenses, Madrid, Spain aut NATIONALLICENCE 700 1- Cohen M. Histopathology Department, Sheffield Children's NHS FT, Sheffield, UK aut NATIONALLICENCE 700 1- Lucena J. Forensic Pathology Service, Institute of Legal Medicine, Seville, Spain aut NATIONALLICENCE 700 1- Van de Voorde W. Department of Forensic Medicine, KU Leuven - University of Leuven, University Hospitals Leuven, 3000, Leuven, Belgium aut NATIONALLICENCE 700 1- Angelini A. Department of Cardiac, Thoracic and Vascular Sciences, University of Padua, Padua, Italy aut NATIONALLICENCE 700 1- Ziyade N. Postmortem Microbiology Laboratory, Council of Forensic Medicine, Istanbul, Turkey aut NATIONALLICENCE 700 1- Saegeman V. Department of Laboratory Medicine, KU Leuven - University Hospitals Leuven, 3000, Leuven, Belgium aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 1045-1057 0934-9723 34:5<1045 2015 34 10096