caa a22 4500 606157719 CHVBK 20210128100615.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s10096-015-2316-y doi (NATIONALLICENCE)springer-10.1007/s10096-015-2316-y Insights into bacterial colonization of intensive care patients' skin: the effect of chlorhexidine daily bathing [Elektronische Daten] [N. Cassir, L. Papazian, P.-E. Fournier, D. Raoult, B. La Scola] Skin is a major reservoir of bacterial pathogens in intensive care unit (ICU) patients. The aim of this study was to assess the skin bacterial richness and diversity in ICU patients and the effect of CHG daily bathing on skin microbiota. Twenty ICU patients were included during an interventional period with CHG daily bathing (n = 10) and a control period (n = 10). At day seven of hospitalization, eight skin swab samples (nares, axillary vaults, inguinal creases, manubrium and back) were taken from each patient. The bacterial identification was performed by microbial culturomics. We used the Shannon index to compare the diversity. We obtained 5,000 colonies that yielded 61 bacterial species (9.15 ± 3.7 per patient), including 15 (24.5%) that had never been cultured from non-pathological human skin before, and three (4.9%) that had never been cultured from human samples before. Notably, Gram-negative bacteria were isolated from all sites. In the water-and-soap group, there was a higher risk of colonization with Gram-negative bacteria (OR = 6.05, 95% CI [1.67-21.90]; P = 0.006). In the CHG group, we observed more patients colonized by sporulating bacteria (9/10 vs. 3/10; P = 0.019) with a reduced skin bacterial richness (P = 0.004) and lower diversity (0.37, 95% CI [0.33; 0.42] vs. 0.50, 95% CI [0.48; 0.52]). Gram-negative bacteria are frequent and disseminated components of the transient skin flora in ICU patients. CHG daily bathing is associated with a reduction in Gram-negative bacteria colonization together with substantial skin microbiota shifts. Springer-Verlag Berlin Heidelberg, 2015 Cassir N. Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Méditerranée-Infection, Aix-Marseille-Université, Marseille, France aut Papazian L. Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Méditerranée-Infection, Aix-Marseille-Université, Marseille, France aut Fournier P.-E Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Méditerranée-Infection, Aix-Marseille-Université, Marseille, France aut Raoult D. Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Méditerranée-Infection, Aix-Marseille-Université, Marseille, France aut La Scola B. Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Méditerranée-Infection, Aix-Marseille-Université, Marseille, France aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 999-1004 0934-9723 34:5<999 2015 34 10096 https://doi.org/10.1007/s10096-015-2316-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2316-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cassir N. Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Méditerranée-Infection, Aix-Marseille-Université, Marseille, France aut NATIONALLICENCE 700 1- Papazian L. Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Méditerranée-Infection, Aix-Marseille-Université, Marseille, France aut NATIONALLICENCE 700 1- Fournier P.-E Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Méditerranée-Infection, Aix-Marseille-Université, Marseille, France aut NATIONALLICENCE 700 1- Raoult D. Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Méditerranée-Infection, Aix-Marseille-Université, Marseille, France aut NATIONALLICENCE 700 1- La Scola B. Unité de Recherche sur les Maladies Infectieuses Tropicales et Emergentes, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Méditerranée-Infection, Aix-Marseille-Université, Marseille, France aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/5(2015-05-01), 999-1004 0934-9723 34:5<999 2015 34 10096