caa a22 4500 606157778 CHVBK 20210128100615.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10096-014-2231-7 doi (NATIONALLICENCE)springer-10.1007/s10096-014-2231-7 Time-kill effect of levofloxacin on multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii [Elektronische Daten] synergism with imipenem and colistin [A. Safarika, I. Galani, A. Pistiki, E. Giamarellos-Bourboulis] In the present study, we challenged the concept that levofloxacin should not be used for the management of ventilator-associated pneumonia (VAP) when minimum inhibitory concentrations (MICs) exceed 2μg/ml. Multidrug-resistant (MDR) and genetically distinct isolates of Pseudomonas aeruginosa (n = 49) and Acinetobacter baumannii (n = 29) from patients with VAP were exposed over time to levofloxacin, imipenem, colistin and their combinations. Synergy between levofloxacin and imipenem was found in 55.3% and between levofloxacin and colistin in 90.9% of isolates of P. aeruginosa within the first 4h of growth. Synergy with imipenem but not with colistin was dependent of the MIC. Synergy between levofloxacin and imipenem was found in 58.6% of isolates of A. baumannii after 24h of growth. Considerable synergy was found between levofloxacin and colistin, reaching 84.8% of isolates of A.baumannii after 6h of growth. Synergy was independent from the MIC. These results create hopes that levofloxacin can be used as combination therapy for infections by MDR bacteria. Springer-Verlag Berlin Heidelberg, 2014 Safarika A. 4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece aut Galani I. 4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece aut Pistiki A. 4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece aut Giamarellos-Bourboulis E. 4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/2(2015-02-01), 317-323 0934-9723 34:2<317 2015 34 10096 https://doi.org/10.1007/s10096-014-2231-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2231-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Safarika A. 4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece aut NATIONALLICENCE 700 1- Galani I. 4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece aut NATIONALLICENCE 700 1- Pistiki A. 4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece aut NATIONALLICENCE 700 1- Giamarellos-Bourboulis E. 4th Department of Internal Medicine, University of Athens, Medical School, Athens, Greece aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/2(2015-02-01), 317-323 0934-9723 34:2<317 2015 34 10096