caa a22 4500 606157808 CHVBK 20210128100615.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10096-014-2242-4 doi (NATIONALLICENCE)springer-10.1007/s10096-014-2242-4 Reducing time to identification of positive blood cultures with MALDI-TOF MS analysis after a 5-h subculture [Elektronische Daten] [A. Verroken, L. Defourny, L. Lechgar, A. Magnette, M. Delmée, Y. Glupczynski] Speeding up the turn-around time of positive blood culture identifications is essential in order to optimize the treatment of septic patients. Several sample preparation techniques have been developed allowing direct matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) identification of positive blood cultures. Yet, the hands-on time restrains their routine workflow. In this study, we evaluated an approach whereby MALDI-TOF MS identification without any additional steps was carried out on short subcultured colonies from positive blood bottles with the objective of allowing results reporting on the day of positivity detection. Over a 7-month period in 2012, positive blood cultures detected by 9am with an automated system were inoculated onto a Columbia blood agar and processed after a 5-h incubation on a MALDI-TOF MicroFlex platform (Bruker Daltonik GmbH). Single-spotted colonies were covered with 1μl formic acid and 1μl matrix solution. The results were compared to the validated identification techniques. A total of 925 positive blood culture bottles (representing 470 bacteremic episodes) were included. Concordant identification was obtained in 727 (81.1%) of the 896 monomicrobial blood cultures, with failure being mostly observed with anaerobes and yeasts. In 17 episodes of polymicrobic bacteremia, the identification of one of the two isolates was achieved in 24/29 (82.7%) positive cultures. Routine implementation of MALDI-TOF MS identification on young positive blood subcultures provides correct results to the clinician in more than 80% of the bacteremic episodes and allows access to identification results on the day of blood culture positivity detection, potentially accelerating the implementation of targeted clinical treatments. Springer-Verlag Berlin Heidelberg, 2014 Verroken A. Institut de recherche expérimentale et clinique (IREC), pôle de microbiologie (MBLG), Université catholique de Louvain, Brussels, Belgium aut Defourny L. Laboratoire de microbiologie, Cliniques universitaires Saint-Luc—Université catholique de Louvain, Brussels, Belgium aut Lechgar L. Laboratoire de microbiologie, Cliniques universitaires Saint-Luc—Université catholique de Louvain, Brussels, Belgium aut Magnette A. Laboratoire de microbiologie, Cliniques universitaires Saint-Luc—Université catholique de Louvain, Brussels, Belgium aut Delmée M. Institut de recherche expérimentale et clinique (IREC), pôle de microbiologie (MBLG), Université catholique de Louvain, Brussels, Belgium aut Glupczynski Y. National Reference Centre for Monitoring of Antimicrobial Resistance in Gram-negative bacteria, CHU Dinant Godinne | UCL Namur, Yvoir, Belgium aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/2(2015-02-01), 405-413 0934-9723 34:2<405 2015 34 10096 https://doi.org/10.1007/s10096-014-2242-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2242-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Verroken A. Institut de recherche expérimentale et clinique (IREC), pôle de microbiologie (MBLG), Université catholique de Louvain, Brussels, Belgium aut NATIONALLICENCE 700 1- Defourny L. Laboratoire de microbiologie, Cliniques universitaires Saint-Luc—Université catholique de Louvain, Brussels, Belgium aut NATIONALLICENCE 700 1- Lechgar L. Laboratoire de microbiologie, Cliniques universitaires Saint-Luc—Université catholique de Louvain, Brussels, Belgium aut NATIONALLICENCE 700 1- Magnette A. Laboratoire de microbiologie, Cliniques universitaires Saint-Luc—Université catholique de Louvain, Brussels, Belgium aut NATIONALLICENCE 700 1- Delmée M. Institut de recherche expérimentale et clinique (IREC), pôle de microbiologie (MBLG), Université catholique de Louvain, Brussels, Belgium aut NATIONALLICENCE 700 1- Glupczynski Y. National Reference Centre for Monitoring of Antimicrobial Resistance in Gram-negative bacteria, CHU Dinant Godinne | UCL Namur, Yvoir, Belgium aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/2(2015-02-01), 405-413 0934-9723 34:2<405 2015 34 10096